The Library
Ex vivo and in vivo regulation of lipocalin-2, a novel adipokine, by insulin
Tools
Tools
Tools
Tan, Bee K., Adya, Raghu, Shan, Xiaoye, Syed, Farhatullah, Levandowski, Krzysztof C., O'Hare, J. Paul and Randeva, Harpal S. (2009) Ex vivo and in vivo regulation of lipocalin-2, a novel adipokine, by insulin. Diabetes Care, Vol.32 (No.1). pp. 129-131. doi:10.2337/dc08-1236 ISSN 0149-5992.
|
Text
WRAP_Tan_Ex_vivo_129.full.pdf - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (267Kb) | Preview |
Official URL: http://dx.doi.org/10.2337/dc08-1236
Abstract
OBJECTIVE - Lipocalin-2, a novel adipokine, has been shown to be elevated in obese, insulin-resistant, and diabetic Subjects. We therefore sought to study the ex vivo and in vivo effects of insulin on lipocalin-2 levels in humans.
RESEARCH DESIGN AND METHODS - We investigated the in vivo effects of insulin (hyperinsulinemia) on circulating lipocalin-2 levels by enzyme-linked immunosorbent assay via a prolonged insulin-glucose infusion. The ex vivo effect of insulin on adipose tissue lipocalin-2 protein production and secretion into conditioned media was assessed by Western blotting and enzyme-linked immunosorbent assay, respectively.
RESULTS - Hyperinsulinemic induction in human subjects significantly increased circulating lipocalin-2 levels (P < 0.01). Also, in omental adipose tissue explants, insulin caused a significant dose-dependent increase in lipocalin-2 protein production and secretion into conditioned media (P < 0.05, P < 0.01, respectively); these effects were negated by both phosphatidylinositol 3-kinase and mitogen-activated protein kinase kinase inhibitions.
CONCLUSIONS - Lipocalin-2 is upregulated by insulin via phosphatidylinositol 3-kinasc and mitogen-activated protein kinase signaling pathways.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QP Physiology R Medicine > RC Internal medicine |
||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||
| Library of Congress Subject Headings (LCSH): | Adipose tissues, Obesity -- Pathophysiology, Insulin resistance | ||||
| Journal or Publication Title: | Diabetes Care | ||||
| Publisher: | American Diabetes Association | ||||
| ISSN: | 0149-5992 | ||||
| Official Date: | January 2009 | ||||
| Dates: |
|
||||
| Volume: | Vol.32 | ||||
| Number: | No.1 | ||||
| Number of Pages: | 3 | ||||
| Page Range: | pp. 129-131 | ||||
| DOI: | 10.2337/dc08-1236 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Date of first compliant deposit: | 13 December 2015 | ||||
| Date of first compliant Open Access: | 13 December 2015 | ||||
| Funder: | General Charities of the City of Coventry (GCCC) |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
