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Activation of NF-E2-related factor-2 reverses biochemical dysfunction of endothelial cells induced by hyperglycemia linked to vascular disease
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Xue, Mingzhan, Qian, Qingwen, Antonysunil, Adaikalakoteswari, Rabbani, Naila, Babaei-Jadidi, Roya and Thornalley, Paul J. (2008) Activation of NF-E2-related factor-2 reverses biochemical dysfunction of endothelial cells induced by hyperglycemia linked to vascular disease. Diabetes, Volume 57 (Number 10). pp. 2809-2817. doi:10.2337/db06-1003 ISSN 0012-1797.
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Official URL: http://dx.doi.org/10.2337/db06-1003
Abstract
OBJECTIVE-Sulforaphane is an activator of transcription factor NF-E2-related factor-2 (nrf2) that regulates gene expression through the promoter antioxidant response element (ARE). Nrf2 regulates the transcription of a battery of protective and metabolic enzymes. The aim of this study was to assess whether activation of nrf2 by sulforaphane in human microvascular endothelial cells prevents metabolic dysfunction in hyperglycemia.
RESEARCH DESIGN AND METHODS-Human microvascular HMEC-1 endothelial cells were incubated in low and high glucose concentrations (5 and 30 mmol/l, respectively), and activation of nrf2 was assessed by nuclear translocation. The effects of sulforaphane on multiple pathways of biochemical dysfunction, increased reactive oxygen species (ROS) formation, hexosamine pathway, protein kinase C (PKC) pathway, and increased formation of methylglyoxal were assessed.
RESULTS-Activation of nrf2 by sulforaphane induced nuclear translocation of nrf2 and increased ARE-linked gene expression, for example, three- to fivefold increased expression of transketolase and glutathione reductase. Hyperglycemia increased the formation of ROS-an effect linked to mitochondrial dysfunction and prevented by sulforaphane. ROS formation was increased further by knockdown of nrf2 and transketolase expression. This also abolished the counteracting effect of sulforaphane, suggesting mediation by nrf2 and related increase of transketolase expression. Sulforaphane also prevented hyperglycemia-induced activation of the hexosamine and PKC pathways and prevented increased cellular accumulation and excretion of the glycating agent methylglyoxal.
CONCLUSIONS-We conclude that activation of nrf2 may prevent biochemical dysfunction and related functional responses of endothelial cells induced by hyperglycemia in which increased expression of transketolase has a pivotal role.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QP Physiology R Medicine > RC Internal medicine |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Vascular endothelial cells, Blood-vessels -- Diseases -- Pathophysiology, Hyperglycemia, Transcription factors, Genetic regulation | ||||
| Journal or Publication Title: | Diabetes | ||||
| Publisher: | American Diabetes Association | ||||
| ISSN: | 0012-1797 | ||||
| Official Date: | October 2008 | ||||
| Dates: |
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| Volume: | Volume 57 | ||||
| Number: | Number 10 | ||||
| Number of Pages: | 9 | ||||
| Page Range: | pp. 2809-2817 | ||||
| DOI: | 10.2337/db06-1003 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Juvenile Diabetes Research Foundation International (JDRF), Wellcome Trust (London, England), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) |
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