Borhan, M. Hossein, Gunn, Nick, Cooper, Abigail, Gulden, Sigrun, Tör, Mahmut, Rimmer, Samuel Roger and Holub, E. B. (2008) WRR4 encodes a TIR-NB-LRR protein that confers broad-spectrum white rust resistance in Arabidopsis thaliana to four physiological races of Albugo candida. Molecular Plant-Microbe Interactions, Vol.21 (No.6). pp. 757-768. doi:10.1094/MPMI-21-6-0757 ISSN 0894-0282.

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Abstract

White blister rust in the Brassicaceae is emerging as a superb model for exploring how plant biodiversity has channeled speciation of biotrophic parasites. The causal agents of white rust across a wide breadth of cruciferous hosts currently are named as variants of a single oomycete species, Albugo candida. The most notable examples include a major group of physiological races that each are economically destructive in a different vegetable or oilseed crop of Brassica juncea (A. candida race 2), B. rapa (race 7), or B. oleracea (race 9); or parasitic on wild crucifers such as Capsella bursa-pastoris (race 4). Arabidopsis thaliana is innately immune to these races of A. candida under natural conditions; however, it commonly hosts its own molecularly distinct subspecies of A. candida (A. candida subsp. arabidopsis). In the laboratory, we have identified several accessions of Arabidopsis thaliana (e.g... Ws-3) that can permit varying degrees of rust development following inoculation with A. candida races 2, 4, and 7, whereas race 9 is universally incompatible in Arabidopsis thaliana and nonrusting resistance is the most prevalent outcome of interactions with the other races. Subtle variation in resistance phenotypes is evident, observed initially with an isolate of A. candida race 4, indicating additional genetic variation. Therefore, we used the race 4 isolate for map-based cloning of the first of many expected white rust resistance (WRR) genes. This gene was designated WRR4 and encodes a cytoplasmic toll-interleukin receptor-like nucleotide-binding leucine-rich repeat receptor-like protein that confers a dominant, broad-spectrum white rust resistance in the Arabidopsis thaliana accession Columbia to representative isolates of A. candida races 2, 4, 7, and 9, as verified by transgenic expression of the Columbia allele in Ws-3. The WRR4 protein requires functional expression of the lipase-like protein EDS1 but not the paralogous protein PAD4, and confers full immunity that masks an underlying nonhypersensitive incompatibility in Columbia to A. candida race 4. This residual incompatibility is independent of functional EDS1.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany Q Science > QR Microbiology > QR180 Immunology S Agriculture > SB Plant culture
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Arabidopsis thaliana, Basidiomycetes, Natural immunity, Albugo candida, Leptosphaeria
Journal or Publication Title:	Molecular Plant-Microbe Interactions
Publisher:	American Phytopathological Society
ISSN:	0894-0282
Official Date:	June 2008
Dates:	Date Event June 2008 Published
Volume:	Vol.21
Number:	No.6
Number of Pages:	12
Page Range:	pp. 757-768
DOI:	10.1094/MPMI-21-6-0757
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access (Creative Commons)
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Canadian Crop Genomics Initiative
Grant number:	D16978 (BBSRC)

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