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Nuclear factor-kappa B induction by visfatin in human vascular endothelial cells : its role in MMP-2/9 production and activation

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Adya, Raghu, Tan, Bee K., Chen, Jing and Randeva, Harpal S. (2008) Nuclear factor-kappa B induction by visfatin in human vascular endothelial cells : its role in MMP-2/9 production and activation. Diabetes Care, Vol.31 (No.4). pp. 758-760. doi:10.2337/dc07-1544 ISSN 0149-5992.

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Official URL: http://dx.doi.org/10.2337/dc07-1544

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Abstract

OBJECTIVE - Visfatin is elevated in obesity and type 2 diabetes and is thought to be an inflammatory mediator within atherosclerotic lesions and to induce gelatinase activity. We ;investigated the activation of nuclear factor-kappa B (NF-kappa B), a well-known proinflammatory transcription factor, by visfatin in endothelial cells.

RESEARCH DESIGN AND METHODS - Human endothelial cells were transfected with pNF-kappa B-Luc plasmid. Using quantitative PCR, Western blot analysis, and gelatin zymography, we studied NF-kappa B signaling in gelatinase-mediated vascular inflammation by visfatin using the NF-kappa B inhibitor BAY 11-7085.

RESULTS - Visfatin significantly increased NF-kappa B transcriptional activity (P < 0.001). We also found a significant inhibition of tumor necrosis factor-alpha JN F-alpha) -induced NF-kappa B activityby visfatin (P < 0.001). Furthermore, the NF-kappa B inhibitor significantly negated visfatin-induced matrix metalloprotemase (MMP)-2/9 mRNA expression, protein levels, and gelatinolytic activity (P < 0.001).

CONCLUSIONS - Visfatin-induced NF-kappa B signaling in human endothelial cells affects the activation of gelatinases MMP-2 and -9, suggesting an important role of visfatin in the pathogenesis of vascular inflammation in obesity and type 2 diabetes.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
R Medicine > RC Internal medicine
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016)
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Vascular endothelium, NF-kappa B (DNA-binding protein), Cardiovascular system -- Diseases
Journal or Publication Title: Diabetes Care
Publisher: American Diabetes Association
ISSN: 0149-5992
Official Date: April 2008
Dates:
DateEvent
April 2008Published
Volume: Vol.31
Number: No.4
Number of Pages: 3
Page Range: pp. 758-760
DOI: 10.2337/dc07-1544
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: General Charities of the City of Coventry

Data sourced from Thomson Reuters' Web of Knowledge

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