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Omentin-1, a novel adipokine, is decreased in overweight insulin-resistant women with polycystic ovary syndrome : ex vivo and in vivo regulation of omentin-1 by insulin and glucose

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Tan, Bee K., Adya, Raghu, Farhatullah, S., Lewandowski, Kris C., O'Hare, J. Paul, Lehnert, Hendrik and Randeva, Harpal S. (2008) Omentin-1, a novel adipokine, is decreased in overweight insulin-resistant women with polycystic ovary syndrome : ex vivo and in vivo regulation of omentin-1 by insulin and glucose. Diabetes, Vol.57 (No.4). pp. 801-808. doi:10.2337/db07-0990 ISSN 0012-1797.

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Official URL: http://dx.doi.org/10.2337/db07-0990

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Abstract

OBJECTIVE - Polycystic ovary syndrome (PCOS) is associated with insulin resistance and obesity. Recent studies have shown that plasma omentin-1 levels decrease with obesity. Currently, no data exist on the relative expression and regulation of omentin-1 in adipose tissue of women with PCOS. The objective of this study was to assess mRNA and protein levels of omentin-1, including circulating omentin-1, in omental adipose tissue of women with PCOS and matched control subjects. Ex vivo and in vivo regulation of adipose tissue omentin-1 was also studied.

RESEARCH DESIGN AND METHODS - Real-time RT-PCR and Western blotting were used to assess mRNA and protein expression of omentin-1. Plasma omentin-1 was measured by enzyme-linked immunosorbent assay. The effects Of D-glucose, insulin, and gonadal and adrenal steroids on adipose tissue omentin-1 were analyzed ex vivo. The in vivo effects of insulin (hyperinsulinemia) on omentin-1 levels were also assessed by a prolonged insulin-glucose infusion.

RESULTS - In addition to decreased plasma omentin-1 levels in women with PCOS (P < 0.05), compared with control subjects, there was significantly lower levels of omentin-1 mRNA (P < 0.01) and protein (P < 0.05) in omental adipose tissue of women with PCOS (P < 0.01). Furthermore, in omental adipose tissue explants, insulin and glucose significantly dose-dependently decreased omentin-1 mRNA expression, protein levels, and secretion into conditioned media (P < 0.05, P < 0.01). Also, hyperinsulinemic induction in healthy subjects significantly reduced plasma omentin-1 levels (P < 0.01).

CONCLUSIONS - Our novel findings reveal that omentin-1 is downregulated by insulin and glucose. These may, in part, explain the decreased omentin-1 levels observed in our overweight women with PCOS.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
R Medicine > RC Internal medicine
R Medicine > RG Gynecology and obstetrics
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016)
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Polycystic ovary syndrome -- Research, Obesity, Insulin resistance, Adipose tissues
Journal or Publication Title: Diabetes
Publisher: American Diabetes Association
ISSN: 0012-1797
Official Date: April 2008
Dates:
DateEvent
April 2008Published
Volume: Vol.57
Number: No.4
Number of Pages: 8
Page Range: pp. 801-808
DOI: 10.2337/db07-0990
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: General Charities of the City of Coventry

Data sourced from Thomson Reuters' Web of Knowledge

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