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The elusive intermediate on the folding pathway of the prion protein
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Jenkins, David C., Sylvester, Ian D. and Pinheiro, Teresa J. T. (2008) The elusive intermediate on the folding pathway of the prion protein. FEBS Journal, Vol.275 (No.6). pp. 1323-1335. doi:10.1111/j.1742-4658.2008.06293.x ISSN 1742-464x.
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Official URL: http://dx.doi.org/10.1111/j.1742-4658.2008.06293.x
Abstract
A key molecular event in prion diseases is the conversion of the cellular conformation of the prion protein (PrPC) to an altered disease-associated form, generally denoted as scrapie isoform (PrPSc). The molecular details of this conformational transition are not fully understood, but it has been suggested that an intermediate on the folding pathway of PrPC may be recruited to form PrPSc. In order to investigate the folding pathway of PrP we designed and expressed two mutants, each possessing a single strategically located tryptophan residue. The secondary structure and folding properties of the mutants were examined. Using conventional analyses of folding transition data determined by fluorescence and CD, and novel phase-diagram analyses, we present compelling evidence for the presence of an intermediate species on the folding pathway of PrP. The potential role of this intermediate in prion conversion is discussed.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | FEBS Journal | ||||
Publisher: | Blackwell | ||||
ISSN: | 1742-464x | ||||
Official Date: | March 2008 | ||||
Dates: |
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Volume: | Vol.275 | ||||
Number: | No.6 | ||||
Number of Pages: | 13 | ||||
Page Range: | pp. 1323-1335 | ||||
DOI: | 10.1111/j.1742-4658.2008.06293.x | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
Data sourced from Thomson Reuters' Web of Knowledge
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