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Measurement and analysis of postsynaptic potentials using a novel voltage-deconvolution method
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Richardson, Magnus J. E. and Silberberg, Gilad (2008) Measurement and analysis of postsynaptic potentials using a novel voltage-deconvolution method. Journal of Neurophysiology, Vol.99 (No.2). pp. 1020-1031. doi:10.1152/jn.00942.2007 ISSN 0022-3077.
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Official URL: http://dx.doi.org/10.1152/jn.00942.2007
Abstract
Accurate measurement of postsynaptic potential amplitudes is a central requirement for the quantification of synaptic strength, dynamics of short-term and long-term plasticity, and vesicle-release statistics. However, the intracellular voltage is a filtered version of the underlying synaptic signal and so a method of accounting for the distortion caused by overlapping postsynaptic potentials must be used. Here a voltage-deconvolution technique is demonstrated that defilters the entire voltage trace to reveal an underlying signal of well-separated synaptic events. These isolated events can be cropped out and reconvolved to yield a set of isolated postsynaptic potentials from which voltage amplitudes may be measured directly-greatly simplifying this common task. The method also has the significant advantage of providing a higher temporal resolution of the dynamics of the underlying synaptic signal. The versatility of the method is demonstrated by a variety of experimental examples, including excitatory and inhibitory connections to neurons with passive membranes and those with activated voltage-gated currents. The deconvolved current-clamp voltage has many features in common with voltage-clamp current measurements. These similarities are analyzed using cable theory and a multicompartment cell reconstruction, as well as direct comparison to voltage-clamp experiments.
| Item Type: | Journal Article | ||||
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| Subjects: | R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Research Centres > Warwick Systems Biology Centre | ||||
| Library of Congress Subject Headings (LCSH): | Neural transmission, Synapses, Synaptic vesicles, Voltage-clamp techniques (Electrophysiology) | ||||
| Journal or Publication Title: | Journal of Neurophysiology | ||||
| Publisher: | American Physiological Society | ||||
| ISSN: | 0022-3077 | ||||
| Official Date: | February 2008 | ||||
| Dates: |
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| Volume: | Vol.99 | ||||
| Number: | No.2 | ||||
| Number of Pages: | 12 | ||||
| Page Range: | pp. 1020-1031 | ||||
| DOI: | 10.1152/jn.00942.2007 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Human Frontier Science Program (Strasbourg, France) (HFSP), Research Councils UK |
Data sourced from Thomson Reuters' Web of Knowledge
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