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The phase change from vegetative to reproductive growth in Agaricus bisporus
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Herman, Bram (2009) The phase change from vegetative to reproductive growth in Agaricus bisporus. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b2318360~S15
Abstract
The phase change from vegetative to reproductive growth in Agaricus
bisporus is a complex process involving changes in morphology at least in part
regulated genetically and influenced by various environmental signals. This work
was aimed at understanding how the morphology changes, and the specific
environmental parameters are involved, and which genes show changes in
transcription during the phase change process in A. bisporus.
Different resources and methodologies were developed and applied to
investigate this process including digital time-lapse photography, genome database
assembly, design, validation and normalisation of a custom oligonucleotide gene
expression microarray and analysis of microarray-generated gene expression profiles
showing the response of this fungus under stimulatory and non-stimulatory
environmental conditions.
Key stages that occur during reproductive differentiation and development
were identified and defined. It was found that temperature and the mushroom
volatile, 1-octen-3-ol, act as an on/off switches as they block specific stages of the
phase change while carbon dioxide acts as a quantitative regulator as high amounts
of this molecule reduce the number of primordia and fruit bodies that develop.
Gene expression profiles were constructed showing the changes in gene
expression in peat-based A. bisporus samples, grown under commercial cultivation
conditions which were designed to stimulate reproductive growth, and experimental
cultivation conditions which were designed to separate out the effects of the three
environmental parameters mentioned previously. It was found that 52 genes were
differentially expressed in A. bisporus during the phase change from vegetative
mycelium into fruit body primordia. A comparison with the gene expression profiles
constructed for the experimental growth conditions, in correlation with
morphological observations enabled the separation of these 52 genes into 3 clusters.
One cluster contained 4 genes that are likely to be involved in the regulation of the
“early” phase change, a second cluster contained 11 genes that are likely to be
involved in the regulation of the “late” phase change and the third cluster contained
37 genes that are likely to be involved with physiological processes supporting the
phase change.
Item Type: | Thesis (PhD) | ||||
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Subjects: | S Agriculture > SB Plant culture | ||||
Library of Congress Subject Headings (LCSH): | Cultivated mushroom -- Research, Mushroom culture -- Research, Fungi -- Genetics | ||||
Official Date: | September 2009 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | Warwick HRI | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Burton, Kerry ; Eastwood, Dan ; Sreenivasaprasad, Prasad | ||||
Sponsors: | Great Britain. Dept. for Environment, Food and Rural Affairs (DEFRA) | ||||
Format of File: | |||||
Extent: | xxi, 216 leaves : col. ill., charts | ||||
Language: | eng |
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