The Library
Hypovirus papain-like protease p29 suppresses RNA silencing in the natural fungal host and in a heterologous plant system
Tools
Segers, Gerrit C., van Wezel, Rene, Zhang, Xuemei , Hong, Yiguo and Nuss, Donald L. (2006) Hypovirus papain-like protease p29 suppresses RNA silencing in the natural fungal host and in a heterologous plant system. Eukaryotic Cell, Vol.5 (No.6). pp. 896-904. doi:10.1128/EC.00373-05 ISSN 1535-9778.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1128/EC.00373-05
Abstract
Virulence-attenuating hypoviruses of the species Cryphonectria hypovirus 1 (CHV1) encode a papain-like protease, p29, that shares similarities with the potyvirus-encoded suppressor of RNA silencing HC-Pro. We now report that hypovirus CRV1-EP713-encoded p29 can suppress RNA silencing in the natural host, the chestnut blight fungus Cryphonectria parasitica. Hairpin RNA-triggered silencing was suppressed in C parasitica strains expressing p29, and transformation of a transgenic green fluorescent protein (GFP)-silenced strain with p29 resulted in an increased number of transformants with elevated GFP expression levels. The CHV1-EP713 p29 protein was also shown to suppress both virus-induced and agroinfiltration-induced RNA silencing and systemic spread of silencing in GFP-expressing transgenic Nicotiana benthamiana line 16c plants. The demonstration that a mycovirus encodes a suppressor of RNA silencing provides circumstantial evidence that RNA silencing in fungi may serve as an antiviral defense mechanism. The observation that a phylogenetically conserved protein of related plant and fungal viruses functions as a suppressor of RNA silencing in both fungi and plants indicates a level of conservation of the mechanisms underlying RNA silencing in these two groups of organisms.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QR Microbiology | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) |
||||
Journal or Publication Title: | Eukaryotic Cell | ||||
Publisher: | American Society for Microbiology | ||||
ISSN: | 1535-9778 | ||||
Official Date: | June 2006 | ||||
Dates: |
|
||||
Volume: | Vol.5 | ||||
Number: | No.6 | ||||
Number of Pages: | 9 | ||||
Page Range: | pp. 896-904 | ||||
DOI: | 10.1128/EC.00373-05 | ||||
Status: | Not Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Public Health Service (US) (PHS) | ||||
Grant number: | GM55981 (PHS) |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |