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High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
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(2011) High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation. Plant Cell, The, Vol.23 (No.3). pp. 873-894. doi:10.1105/tpc.111.083345 ISSN 1040-4651.
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PDF (See also supplemental data at http://www.plantcell.org/content/23/3/873/suppl/DC1)
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Official URL: http://dx.doi.org/10.1105/tpc.111.083345
Abstract
Leaf senescence is an essential developmental process that impacts dramatically on crop yields and involves altered
regulation of thousands of genes and many metabolic and signaling pathways, resulting in major changes in the leaf. The
regulation of senescence is complex, and although senescence regulatory genes have been characterized, there is little
information on how these function in the global control of the process. We used microarray analysis to obtain a highresolution
time-course profile of gene expression during development of a single leaf over a 3-week period to senescence.
A complex experimental design approach and a combination of methods were used to extract high-quality replicated data
and to identify differentially expressed genes. The multiple time points enable the use of highly informative clustering to
reveal distinct time points at which signaling and metabolic pathways change. Analysis of motif enrichment, as well
as comparison of transcription factor (TF) families showing altered expression over the time course, identify clear groups
of TFs active at different stages of leaf development and senescence. These data enable connection of metabolic
processes, signaling pathways, and specific TF activity, which will underpin the development of network models to
elucidate the process of senescence.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QK Botany | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Research Centres > Warwick Systems Biology Centre |
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Library of Congress Subject Headings (LCSH): | Leaves -- Growth, Leaves -- Physiology, Plant genetic regulation, Plant gene expression | ||||
Journal or Publication Title: | Plant Cell, The | ||||
Publisher: | American Society of Plant Biologists | ||||
ISSN: | 1040-4651 | ||||
Official Date: | March 2011 | ||||
Dates: |
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Volume: | Vol.23 | ||||
Number: | No.3 | ||||
Page Range: | pp. 873-894 | ||||
DOI: | 10.1105/tpc.111.083345 | ||||
Status: | Peer Reviewed | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||
Grant number: | BB/ F005806/1 (BBSRC) |
Data sourced from Thomson Reuters' Web of Knowledge
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