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CO2-dependent opening of an inwardly rectifying K+ channel
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Huckstepp, Robert T. R. and Dale, Nicholas (2011) CO2-dependent opening of an inwardly rectifying K+ channel. Pfluegers Archiv, Vol.461 (No.3). pp. 337-344. doi:10.1007/s00424-010-0916-z ISSN 0031-6768.
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Official URL: http://dx.doi.org/10.1007/s00424-010-0916-z
Abstract
CO2 chemosensing is a vital function for the
maintenance of life that helps to control acid–base balance.
Most studies have reported that CO2 is measured via its
proxy, pH. Here we report an inwardly rectifying channel,
in outside-out excised patches from HeLa cells that was
sensitive to modest changes in PCO2 under conditions of
constant extracellular pH. As PCO2 increased, the open
probability of the channel increased. The single-channel
currents had a conductance of 6.7 pS and a reversal
potential of –70 mV, which lay between the K+ and Cl–
equilibrium potentials. This reversal potential was shifted
by +61 mV following a tenfold increase in extracellular
[K+] but was insensitive to variations of extracellular [Cl–].
The single-channel conductance increased with extracellular
[K+]. We propose that this channel is a member of the
Kir family. In addition to this K+ channel, we found that
many of the excised patches also contained a conductance
carried via a Cl–-selective channel. This CO2-sensitive Kir
channel may hyperpolarize excitable cells and provides a
potential mechanism for CO2-dependent inhibition during
hypercapnia.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QP Physiology | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Chemical senses, Carbon dioxide, Blood gases, Biological control systems | ||||
Journal or Publication Title: | Pfluegers Archiv | ||||
Publisher: | Springer | ||||
ISSN: | 0031-6768 | ||||
Official Date: | 14 January 2011 | ||||
Dates: |
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Volume: | Vol.461 | ||||
Number: | No.3 | ||||
Page Range: | pp. 337-344 | ||||
DOI: | 10.1007/s00424-010-0916-z | ||||
Status: | Peer Reviewed | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Medical Research Council (Great Britain) (MRC) |
Data sourced from Thomson Reuters' Web of Knowledge
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