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Escherichia coli σ70 senses sequence and conformation of the promoter spacer region
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Singh, Shivani S., Typas, Athanasios, Hengge, Regine and Grainger, David C. (2011) Escherichia coli σ70 senses sequence and conformation of the promoter spacer region. Nucleic Acids Research, Vol.39 (No.12). pp. 5109-5118. doi:10.1093/nar/gkr080 ISSN 1362-4962.
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Official URL: http://dx.doi.org/10.1093/nar/gkr080
Abstract
In bacteria, promoter identification by RNA polymerase
is mediated by a dissociable p factor. The
housekeeping σ70 factor of Escherichia coli recognizes
two well characterized DNA sequence
elements, known as the ‘-10’ and ‘-35’ hexamers.
These elements are separated by ‘spacer’ DNA, the
sequence of which is generally considered unimportant.
Here, we use a combination of bioinformatics,
genetics and biochemistry to show that σ70
can sense the sequence and conformation of the
promoter spacer region. Our data illustrate how
alterations in spacer region sequence can increase
promoter activity. This stimulatory effect requires
σ70 side chain R451, which is located in close proximity
to the non-template strand at promoter
position -18. Conversely, R451 is not required to
mediate transcriptional stimulation by improvement
of the -10 element. Mutation of σ70 residue
R451, which is highly conserved, results in reduced
growth rate, consistent with a central role in
promoter recognition.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Escherichia coli -- Genetics, RNA polymerases, Promoters (Genetics) | ||||
Journal or Publication Title: | Nucleic Acids Research | ||||
Publisher: | Oxford University Press | ||||
ISSN: | 1362-4962 | ||||
Official Date: | 11 March 2011 | ||||
Dates: |
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Volume: | Vol.39 | ||||
Number: | No.12 | ||||
Page Range: | pp. 5109-5118 | ||||
DOI: | 10.1093/nar/gkr080 | ||||
Status: | Peer Reviewed | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 16 December 2015 | ||||
Date of first compliant Open Access: | 16 December 2015 | ||||
Funder: | Wellcome Trust (London, England), Deutsche Forschungsgemeinschaft (DFG) | ||||
Grant number: | He1556/12-1 (DFG) |
Data sourced from Thomson Reuters' Web of Knowledge
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