Kerr, Gary W., Sarkar, Sourav, Tibbles, Katherine L., Petronczki, Mark, Millar, Jonathan B. A. and Arumugam, Prakash (2011) Meiotic nuclear divisions in budding yeast require PP2ACdc55-mediated antagonism of Net1 phosphorylation by Cdk. The Journal of Cell Biology, Vol.193 (No.7). pp. 1157-1166. doi:10.1083/jcb.201103019

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Abstract

During meiosis, one round of deoxyribonucleic acid replication is followed by two rounds of nuclear division. In Saccharomyces cerevisiae, activation of the Cdc14 early anaphase release (FEAR) network is required for exit from meiosis I but does not lead to the activation of origins of replication. The precise mechanism of how FEAR regulates meiosis is not understood. In this paper, we report that premature activation of FEAR during meiosis caused by loss of protein phosphatase PP2ACdc55 activity blocks bipolar spindle assembly and nuclear divisions. In cdc55 meiotic null (cdc55-mn) cells, the cyclin-dependent kinase (Cdk)–counteracting phosphatase Cdc14 was released prematurely from the nucleolus concomitant with hyperphosphorylation of its nucleolar anchor protein Net1. Crucially, a mutant form of Net1 that lacks six Cdk phosphorylation sites rescued the meiotic defect of cdc55-mn cells. Expression of a dominant mutant allele of CDC14 mimicked the cdc55-mn phenotype. We propose that phosphoregulation of Net1 by PP2ACdc55 is essential for preventing precocious exit from meiosis I.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH426 Genetics
Divisions:	Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science > Life Sciences (2010- ) Faculty of Science > Centre for Systems Biology Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Meiosis, Saccharomyces cerevisiae -- Genetics
Journal or Publication Title:	The Journal of Cell Biology
Publisher:	Rockefeller University Press
ISSN:	0021-9525
Official Date:	27 June 2011
Dates:	Date Event 27 June 2011 Published
Date of first compliant deposit:	17 December 2015
Volume:	Vol.193
Number:	No.7
Page Range:	pp. 1157-1166
DOI:	10.1083/jcb.201103019
Status:	Peer Reviewed
Access rights to Published version:	Restricted or Subscription Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
Grant number:	BB/G00353X/1 (BBSRC)

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