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Meiotic nuclear divisions in budding yeast require PP2ACdc55-mediated antagonism of Net1 phosphorylation by Cdk
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Kerr, Gary W., Sarkar, Sourav, Tibbles, Katherine L., Petronczki, Mark, Millar, Jonathan B. A. and Arumugam, Prakash (2011) Meiotic nuclear divisions in budding yeast require PP2ACdc55-mediated antagonism of Net1 phosphorylation by Cdk. The Journal of Cell Biology, Vol.193 (No.7). pp. 1157-1166. doi:10.1083/jcb.201103019 ISSN 0021-9525.
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WRAP_Kerr_0170657-lb-210711_j_cell_biol-2011-kerr-1157-66.pdf - Published Version - Requires a PDF viewer. Download (2771Kb) |
Official URL: http://dx.doi.org/10.1083/jcb.201103019
Abstract
During meiosis, one round of deoxyribonucleic acid replication is followed by two rounds of nuclear division. In Saccharomyces cerevisiae, activation of the Cdc14 early anaphase release (FEAR) network is required for exit from meiosis I but does not lead to the activation of origins of replication. The precise mechanism of how FEAR regulates meiosis is not understood. In this paper, we report that premature activation of FEAR during meiosis caused by loss of protein phosphatase PP2ACdc55 activity blocks bipolar spindle assembly and nuclear divisions. In cdc55 meiotic null (cdc55-mn) cells, the cyclin-dependent kinase (Cdk)–counteracting phosphatase Cdc14 was released prematurely from the nucleolus concomitant with hyperphosphorylation of its nucleolar anchor protein Net1. Crucially, a mutant form of Net1 that lacks six Cdk phosphorylation sites rescued the meiotic defect of cdc55-mn cells. Expression of a dominant mutant allele of CDC14 mimicked the cdc55-mn phenotype. We propose that phosphoregulation of Net1 by PP2ACdc55 is essential for preventing precocious exit from meiosis I.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Research Centres > Warwick Systems Biology Centre Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Meiosis, Saccharomyces cerevisiae -- Genetics | ||||
Journal or Publication Title: | The Journal of Cell Biology | ||||
Publisher: | Rockefeller University Press | ||||
ISSN: | 0021-9525 | ||||
Official Date: | 27 June 2011 | ||||
Dates: |
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Volume: | Vol.193 | ||||
Number: | No.7 | ||||
Page Range: | pp. 1157-1166 | ||||
DOI: | 10.1083/jcb.201103019 | ||||
Status: | Peer Reviewed | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 17 December 2015 | ||||
Date of first compliant Open Access: | 17 December 2015 | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||
Grant number: | BB/G00353X/1 (BBSRC) |
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