The Library
An external matrix assisted laser desorption ionization source for flexible FT-ICR mass spectrometry imaging with internal calibration on adjacent samples
Tools
Smith, Donald F., Aizikov, Konstantin, Duursma, Marc C., Giskes, Frans, Spaanderman, Dirk-Jan, McDonnell, Liam A., O’Connor, Peter B. and Heeren, Ron M. A. (2011) An external matrix assisted laser desorption ionization source for flexible FT-ICR mass spectrometry imaging with internal calibration on adjacent samples. Journal of The American Society for Mass Spectrometry, Vol.22 (No.1). pp. 130-137. doi:10.1007/s13361-010-0003-x ISSN 1044-0305.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1007/s13361-010-0003-x
Abstract
We describe the construction and application of a new MALDI source for FT-ICR mass spectrometry imaging. The source includes a translational X-Y positioning stage with a 10×10 cm range of motion for analysis of large sample areas, a quadrupole for mass selection, and an external octopole ion trap with electrodes for the application of an axial potential gradient for controlled ion ejection. An off-line LC MALDI MS/MS run demonstrates the utility of the new source for data- and position-dependent experiments. A FT-ICR MS imaging experiment of a coronal rat brain section yields ∼200 unique peaks from m/z 400-1100 with corresponding mass-selected images. Mass spectra from every pixel are internally calibrated with respect to polymer calibrants collected from an adjacent slide.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Journal or Publication Title: | Journal of The American Society for Mass Spectrometry | ||||
Publisher: | Springer New York LLC | ||||
ISSN: | 1044-0305 | ||||
Official Date: | January 2011 | ||||
Dates: |
|
||||
Volume: | Vol.22 | ||||
Number: | No.1 | ||||
Number of Pages: | 8 | ||||
Page Range: | pp. 130-137 | ||||
DOI: | 10.1007/s13361-010-0003-x | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Nederlandseorganisatie voor Wetenschappelijk Onderzoek (NWO), European Union (EU), Netherlands Proteomics Centre | ||||
Grant number: | LSHG-CT-2005-5181194 (EU) |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |