The Library
Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases
Tools
Tools
Tools
Jaqaman, K., King, E. M., Amaro, A. C., Winter, J. R., Dorn, J. F., Elliott, Hunter L., Mchedlishvili, N., McClelland, S. E., Porter, I. M., Posch, M., Toso, A., Danuser, G., McAinsh, Andrew D., Meraldi, P. and Swedlow, Jason (2010) Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases. Journal of Cell Biology, Vol.188 (No.5). pp. 665-679. doi:10.1083/jcb.200909005 ISSN 0021-9525.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1083/jcb.200909005
Abstract
During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores, commonly referred to as breathing. We developed a live cell imaging assay combined with computational image analysis to quantify the properties and dynamics of sister kinetochores in three dimensions. We show that baseline oscillation and breathing speeds in late prometaphase and metaphase are set by microtubule depolymerases, whereas oscillation and breathing periods depend on the stiffness of the mechanical linkage between sisters. Metaphase plates become thinner as cells progress toward anaphase as a result of reduced oscillation speed at a relatively constant oscillation period. The progressive slowdown of oscillation speed and its coupling to plate thickness depend nonlinearly on the stiffness of the mechanical linkage between sisters. We propose that metaphase plate formation and thinning require tight control of the state of the mechanical linkage between sisters mediated by centromeric chromatin and cohesion.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QP Physiology |
||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||
| Library of Congress Subject Headings (LCSH): | Microtubules, Cells -- Motility, Kinesin, Cell separation | ||||
| Journal or Publication Title: | Journal of Cell Biology | ||||
| Publisher: | Rockefeller University Press | ||||
| ISSN: | 0021-9525 | ||||
| Official Date: | 8 March 2010 | ||||
| Dates: |
|
||||
| Volume: | Vol.188 | ||||
| Number: | No.5 | ||||
| Page Range: | pp. 665-679 | ||||
| DOI: | 10.1083/jcb.200909005 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Marie Curie Cancer Care, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung [Swiss National Science Foundation], Eidgenössische Technische Hochschule Zürich, National Institutes of Health (U.S.) (NIH) | ||||
| Grant number: | GM68956 (NIH) |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
