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Semi-automated Acanthamoeba polyphaga detection and computation of Salmonella typhimurium concentration in spatio-temporal images
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Tsibidis, George D., Burroughs, Nigel John, Gaze, William H. and Wellington, E. M. H. (2011) Semi-automated Acanthamoeba polyphaga detection and computation of Salmonella typhimurium concentration in spatio-temporal images. Micron, Vol.42 (No.8). pp. 911-920. doi:10.1016/j.micron.2011.06.010 ISSN 0968-4328.
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Official URL: http://dx.doi.org/10.1016/j.micron.2011.06.010
Abstract
Interaction between bacteria and protozoa is an increasing area of interest, however there are a few systems that allow extensive observation of the interactions. A semi-automated approach is proposed to analyse a large amount of experimental data and avoid a time demanding manual object classification. We examined a surface system consisting of non nutrient agar with a uniform bacterial lawn that extended over the agar surface, and a spatially localised central population of amoebae. Location and identification of protozoa and quantification of bacteria population are performed by the employment of image analysis techniques in a series of spatial images. The quantitative tools are based on intensity thresholding, or on probabilistic models. To accelerate organism identification, correct classification errors and attain quantitative details of all objects a custom written Graphical User Interfaces has also been developed.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology T Technology > TA Engineering (General). Civil engineering (General) |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Research Centres > Warwick Systems Biology Centre |
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| Library of Congress Subject Headings (LCSH): | Image analysis, Diagnostic microbiology , Microscopy, Acanthamoeba, Salmonella typhimurium, Computational biology | ||||
| Journal or Publication Title: | Micron | ||||
| Publisher: | Pergamon-Elsevier Science LTD | ||||
| ISSN: | 0968-4328 | ||||
| Official Date: | December 2011 | ||||
| Dates: |
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| Volume: | Vol.42 | ||||
| Number: | No.8 | ||||
| Number of Pages: | 10 | ||||
| Page Range: | pp. 911-920 | ||||
| DOI: | 10.1016/j.micron.2011.06.010 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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