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Short communication : preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk
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Smith, Edward M., Monaghan, E. M. (Emma M.), Huntley, Selene J. and Green, Laura E. (2011) Short communication : preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk. Journal of Dairy Science, Vol.94 (No.10). pp. 4850-4855. doi:10.3168/jds.2010-4076 ISSN 00220302.
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Official URL: http://dx.doi.org/10.3168/jds.2010-4076
Abstract
The objective of this study was to investigate the recovery of bacteria from ewe milk after freezing for 4 or 8 weeks with and without the addition of glycerol as a cryopreservant. A total of 50 udder-half milk samples with a known range of bacterial species were selected, stored and analyzed in 5 treatment-groups: time zero, frozen for 4 weeks with, and without, glycerol; and frozen for 8 weeks with, and without, glycerol. There was a lower recovery of all bacterial species studied after freezing. Samples containing fewer than 100 cfu/mL came from ewes with a lower somatic cell count and were more likely to be bacteriologically negative after freezing than those above this threshold. The addition of glycerol increased recovery of Gram-negative bacteria after freezing, although this requires further study to draw strong conclusions. The effects on Gram-positive species were inconsistent. We conclude that whilst the addition of glycerol had a small beneficial impact on the sensitivity of detection of bacteria from frozen sheep milk, sensitivity was highest in cultures from fresh milk.
Item Type: | Journal Article | ||||
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Alternative Title: | Preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk | ||||
Subjects: | Q Science > QR Microbiology S Agriculture > SF Animal culture |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Sheep milk -- Analysis, Glycerin, Cryobiology, Mastitis | ||||
Journal or Publication Title: | Journal of Dairy Science | ||||
Publisher: | Elsevier | ||||
ISSN: | 00220302 | ||||
Official Date: | October 2011 | ||||
Dates: |
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Volume: | Vol.94 | ||||
Number: | No.10 | ||||
Page Range: | pp. 4850-4855 | ||||
DOI: | 10.3168/jds.2010-4076 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 18 December 2015 | ||||
Date of first compliant Open Access: | 18 December 2015 | ||||
Funder: | Wellcome Trust (London, England) |
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