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Crystallization and preliminary X-ray analysis of a D-alanyl-D-alanine ligase (EcDdlB) from Escherichia coli
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Batson, Sarah, Rea, Dean, Fülöp, Vilmos and Roper, David I. (2010) Crystallization and preliminary X-ray analysis of a D-alanyl-D-alanine ligase (EcDdlB) from Escherichia coli. Journal of Experimental Psychology: Human Perception and Performance, Vol.66 (No.4). pp. 405-408. doi:10.1107/S1744309110003970
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Official URL: http://dx.doi.org/10.1107/S1744309110003970
Abstract
A recombinant form of Escherichia coli DdlB (EcDdlB) has been prepared and cocrystallized with ADP and D-alanyl-D-alanine to represent the ternary complex of EcDdlB. Furthermore, EcDdlB has been cocrystallized under the same conditions with the ligands ATP and D-alanyl-D-alanine, representing the product-inhibited complex. The crystals belonged to space group P212121, with unit-cell parameters a = 53.0, b = 97.6, c = 109.5 Å and a = 51.2, b = 97.8, c = 110.1 Å, respectively, and both contained two molecules in the asymmetric unit. Complete data sets were collected to 1.5 and 1.4 Å resolution, respectively, from single crystals under cryogenic conditions using synchrotron radiation.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QR Microbiology | ||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
| Library of Congress Subject Headings (LCSH): | Escherichia coli, Peptidoglycans -- Synthesis | ||||
| Journal or Publication Title: | Journal of Experimental Psychology: Human Perception and Performance | ||||
| Publisher: | American Psychological Association | ||||
| ISSN: | 0096-1523 | ||||
| Official Date: | April 2010 | ||||
| Dates: |
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| Volume: | Vol.66 | ||||
| Number: | No.4 | ||||
| Page Range: | pp. 405-408 | ||||
| DOI: | 10.1107/S1744309110003970 | ||||
| Status: | Peer Reviewed | ||||
| Access rights to Published version: | Open Access | ||||
| Funder: | University of Warwick, Medical Research Council (Great Britain) (MRC), Wellcome Trust (London, England), Advantage West Midlands (AWM), Birmingham Science City | ||||
| Grant number: | G500643 (MRC), G0600801 (MRC), 071998 (Wellcome), 068598 (Wellcome) |
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