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Crystallization and preliminary X-ray analysis of a D-alanyl-D-alanine ligase (EcDdlB) from Escherichia coli

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Batson, Sarah, Rea, Dean, Fülöp, Vilmos and Roper, David I. (2010) Crystallization and preliminary X-ray analysis of a D-alanyl-D-alanine ligase (EcDdlB) from Escherichia coli. Journal of Experimental Psychology: Human Perception and Performance, Vol.66 (No.4). pp. 405-408. doi:10.1107/S1744309110003970 ISSN 0096-1523.

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Official URL: http://dx.doi.org/10.1107/S1744309110003970

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Abstract

A recombinant form of Escherichia coli DdlB (EcDdlB) has been prepared and cocrystallized with ADP and D-alanyl-D-alanine to represent the ternary complex of EcDdlB. Furthermore, EcDdlB has been cocrystallized under the same conditions with the ligands ATP and D-alanyl-D-alanine, representing the product-inhibited complex. The crystals belonged to space group P212121, with unit-cell parameters a = 53.0, b = 97.6, c = 109.5 Å and a = 51.2, b = 97.8, c = 110.1 Å, respectively, and both contained two molecules in the asymmetric unit. Complete data sets were collected to 1.5 and 1.4 Å resolution, respectively, from single crystals under cryogenic conditions using synchrotron radiation.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH): Escherichia coli, Peptidoglycans -- Synthesis
Journal or Publication Title: Journal of Experimental Psychology: Human Perception and Performance
Publisher: American Psychological Association
ISSN: 0096-1523
Official Date: April 2010
Dates:
DateEvent
April 2010Published
Volume: Vol.66
Number: No.4
Page Range: pp. 405-408
DOI: 10.1107/S1744309110003970
Status: Peer Reviewed
Access rights to Published version: Open Access (Creative Commons)
Funder: University of Warwick, Medical Research Council (Great Britain) (MRC), Wellcome Trust (London, England), Advantage West Midlands (AWM), Birmingham Science City
Grant number: G500643 (MRC), G0600801 (MRC), 071998 (Wellcome), 068598 (Wellcome)

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