Ho, Thien, Wang, Liang, Huang, Linfeng, Li, Zhigang, Pallett, Denise W., Dalmay, Tamas, Ohshima, Kazusato, Walsh, John A. and Wang, Hui (2010) Nucleotide bias of DCL and AGO in plant anti-virus gene silencing. Protein & Cell, Vol.1 (No.9). pp. 847-858. doi:10.1007/s13238-010-0100-4 ISSN 1674-8018 .

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Abstract

Plant Dicer-like (DCL) and Argonaute (AGO) are the key enzymes involved in anti-virus post-transcriptional gene silencing (AV-PTGS). Here we show that AV-PTGS exhibited nucleotide preference by calculating a relative AV-PTGS efficiency on processing viral RNA substrates. In comparison with genome sequences of dicot-infecting Turnip mosaic virus (TuMV) and monocot-infecting Cocksfoot streak virus (CSV), viral-derived small interfering RNAs (vsiRNAs) displayed positive correlations between AV-PTGS efficiency and G+C content (GC%). Further investigations on nucleotide contents revealed that the vsiRNA populations had G-biases. This finding was further supported by our analyses of previously reported vsiRNA populations in diverse plant-virus associations, and AGO associated Arabidopsis endogenous siRNA populations, indicating that plant AGOs operated with G-preference. We further propose a hypothesis that AV-PTGS imposes selection pressure(s) on the evolution of plant viruses. This hypothesis was supported when potyvirus genomes were analysed for evidence of GC elimination, suggesting that plant virus evolution to have low GC% genomes would have a unique function, which is to reduce the host AV-PTGS attack during infections.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Plant enzymes, Plant gene silencing, Plant viruses -- Genetics
Journal or Publication Title:	Protein & Cell
Publisher:	Springer
ISSN:	1674-8018
Official Date:	September 2010
Dates:	Date Event September 2010 Published
Volume:	Vol.1
Number:	No.9
Page Range:	pp. 847-858
DOI:	10.1007/s13238-010-0100-4
Status:	Peer Reviewed
Access rights to Published version:	Restricted or Subscription Access
Funder:	Natural Environment Research Council (Great Britain) (NERC), Vietnam. Bộ giáo dục và đào tạo [Ministry of Education and Training], Centre for Ecology and Hydrology (Great Britain) (CEH)
Grant number:	322/QD-TTg (Vietnam), NER/A/S/2003/00547 (NERC), NER/A/S/2003/00548 (NERC), NE/E008933/1 (NERC), C02875 (CEH)

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