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Assimilatory potential of Helicoverpa armigera reared on host (Chickpea) and nonhost (Cassia tora) diets
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Dawkar, Vishal V., Chikate, Yojana R., Gupta, Vidya S., Slade, Susan E. and Giri, Ashok P. (2011) Assimilatory potential of Helicoverpa armigera reared on host (Chickpea) and nonhost (Cassia tora) diets. Journal of Proteome Research, Vol.10 (No.11). pp. 5128-5138. doi:10.1021/pr200591m ISSN 1535-3893.
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Official URL: http://dx.doi.org/10.1021/pr200591m
Abstract
Adaptation to plant allelochemicals is a crucial aspect of herbivore chemical ecology. To understand an insect ecology, we studied an effect of nonhost Cassia tora seed-based diet (Ct) on growth, development, and molecular responses in Helicoverpa armigera. We employed a comparative approach to investigate the proteomic differences in gut, hemolymph, and frass of H. armigera reared on a normal (chickpea seed-based, Cp) and Ct diet. In this study, a total of 46 proteins were identified by nano-LC-MS(E). Among them, 17 proteins were up-regulated and 29 proteins were down-regulated when larvae were exposed to the Ct diet. Database searches combined with GO analysis revealed that gut proteases engrossed in digestion, proteins crucial for immunity, adaptive responses to stress, and detoxification were down-regulated in the Ct fed larvae. Proteins identified in H. armigera hemolymph were found to be involved in defense mechanisms. Moreover, proteins found in frass of the Ct fed larvae were observed to participate in energy metabolism. Biochemical and quantitative real-time PCR analysis of selected candidate proteins showed differential gene expression patterns and corroborated with the proteomic data. Our results suggest that the Ct diet could alter expression of proteins related to digestion, absorption of nutrients, adaptation, defense mechanisms, and energy metabolism in H. armigera.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Helicoverpa armigera | ||||
Journal or Publication Title: | Journal of Proteome Research | ||||
Publisher: | American Chemical Society | ||||
ISSN: | 1535-3893 | ||||
Official Date: | November 2011 | ||||
Dates: |
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Volume: | Vol.10 | ||||
Number: | No.11 | ||||
Page Range: | pp. 5128-5138 | ||||
DOI: | 10.1021/pr200591m | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Council of Scientific & Industrial Research (India) (CSIR) | ||||
Grant number: | NWP 0003 (CSIR) |
Data sourced from Thomson Reuters' Web of Knowledge
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