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High-resolution solid-state NMR structure of a 17.6 kDa protein
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Bertini, Ivano, Bhaumik, Anusarka, De Paëpe, Gaël, Griffin, Robert G., Lelli, Moreno, Lewandowski, Józef R. and Luchinat, C. (2010) High-resolution solid-state NMR structure of a 17.6 kDa protein. Journal of the American Chemical Society, Vol.132 (No.3). pp. 1032-1040. doi:10.1021/ja906426p ISSN 0002-7863.
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Official URL: http://dx.doi.org/10.1021/ja906426p
Abstract
The use of pseudocontact shifts arising from paramagnetic metal ions in a microcrystalline protein sample is proposed as a strategy to obtain unambiguous signal assignments in solid-state NMR spectra enabling distance extraction for protein structure calculation. With this strategy, 777 unambiguous (281 sequential, 217 medium-range, and 279 long-range) distance restraints could be obtained from PDSD, DARR, CHHC, and the recently introduced PAR and PAIN-CP solid-state experiments for the cobalt(II)substituted catalytic domain of matrix metalloproteinase 12 (159 amino acids, 17.6 kDa). The obtained structure is a high resolution one, with backbone rmsd of 1.0 +/- 0.2 angstrom, and is in good agreement with the X-ray structure (rmsd to X-ray 1.3 angstrom). The proposed strategy, which may be generalized for nonmetalloproteins with the use of paramagnetic tags, represents a significant step ahead in protein structure determination using solid-state NMR.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Library of Congress Subject Headings (LCSH): | Nuclear magnetic resonance spectroscopy, Solid state chemistry, Proteins -- Structure, Proteins -- Analysis | ||||
Journal or Publication Title: | Journal of the American Chemical Society | ||||
Publisher: | American Chemical Society | ||||
ISSN: | 0002-7863 | ||||
Official Date: | 27 January 2010 | ||||
Dates: |
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Volume: | Vol.132 | ||||
Number: | No.3 | ||||
Page Range: | pp. 1032-1040 | ||||
DOI: | 10.1021/ja906426p | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | European Commission (EC), Italy. Ministero dell'istruzione, dell'università e della ricerca (MIUR), Ente Cassa di risparmio di Firenze | ||||
Grant number: | 201640(SFMET) (EC), LSHB-CT-2005-019102 (EC), 026145 (EU-NMR JRA2 and JRA3) (EC), Bio-DNP 011721 (EC), SPINE2-COMPLEXES 031220 (EC), RBAU013NSB (MIUR), RBLA032M7 (MIUR), RBIP06LSS2 (MIUR) |
Data sourced from Thomson Reuters' Web of Knowledge
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