Rusholme, Rachel L., Higgins, Erin E., Walsh, John A. and Lydiate, Derek J. (2007) Broad-spectrum resistance to turnip mosaic virus in Brassica rapa (Chinese cabbage). In: International Conference, Genetic control of plant pathogenic viruses and their vectors: Towards new resistance strategies, El Puerto de Santa Maria, Cadiz, Spain, 23-27 Nov 2008. Published in: Proceedings of the Genetic control of plant pathogenic viruses and their vectors: Towards new resistance strategies doi:10.1099/vir.0.83194-0

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Abstract

TuMV is a member of the Potyvirus genus, infects a wide range of cultivated plant species and causes significant
economic losses in Brassica crops (Shattuck, 1992). It is a positive strand RNA virus (genome comprises 9830-
9835 nucleotides) and is the subject of advanced molecular characterisation in terms of its interaction with
brassicas (Walsh & Jenner, 2002). Plant genes for resistance to TuMV have been mapped in lettuce (Tu,
Robbins et al., 1994), B. napus (TuRB01, Walsh et al., 1999; TuRB03, Hughes et al., 2003; TuRB04 and
TuRB05, Walsh & Lydiate, unpublished) and B. rapa (TuRB01b, Rusholme et al., unpublished). All these
brassica genes are dominant R genes that control resistance to narrow spectra of TuMV isolates; the viral
avirulence determinants for these genes have been identified (Jenner et al., 2000; Walsh et al., 2002; Jenner et
al., 2002; Jenner et al., 2003).
The B. rapa line RLR22 is resistant to a diverse range of TuMV isolates from different regions of the world,
different pathotypes, different serotypes and different genetic groups (Walsh et al., 2002; Rusholme et al., 2007).
A B. rapa genetic map based on 213 marker loci segregating in 120 first backcross (B1) individuals was
established. B1 individuals were self-pollinated to produce B1S1 families. The existence of two loci controlling
resistance to TuMV isolate CDN 1 was established from contrasting patterns of segregation for resistance and
susceptibility in the B1S1 families. The first gene recessive TuMV resistance 01 (retr01) had a recessive allele for
resistance, was located on the upper portion of chromosome R4 and was epistatic to the second gene. The
second gene Conditional TuMV Resistance 01 (ConTR01) possessed a dominant allele for resistance and was
located on the upper portion of chromosome R8. These genes also controlled resistance to TuMV isolate CZE 1
and might be sufficient to explain the broad-spectrum resistance of RLR22. The dominant resistance gene,
ConTR01, was coincident with one of the three eukaryotic initiation factor 4E (eIF4E) loci of B. rapa and the
recessive resistance gene retr01 was apparently co-incident with one of the three loci of eIF(iso)4E in the Agenome of B. napus and therefore, by inference, in the B. rapa genome. This suggested a mode of action for the
resistance that is based on denying the viral RNA access to the translation initiation complex of the plant host.
retr01 is the first example of a recessive resistance gene mapped in a brassica.

Item Type:	Conference Item (UNSPECIFIED)
Subjects:	T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Journal or Publication Title:	Proceedings of the Genetic control of plant pathogenic viruses and their vectors: Towards new resistance strategies
Official Date:	November 2007
Dates:	Date Event November 2007 Published
Number of Pages:	10
DOI:	10.1099/vir.0.83194-0
Status:	Not Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Version or Related Resource:	Also presented at:C.E. Jenner, R.L. Rusholme, E.E. Higgins, D.J. Lydiate, G. Barker and J.A. Walsh (2008). Broad-spectrum resistance to Turnip mosaic virus (TuMV) in Brassica rapa (Chinese cabbage). 3rd International Conference of the International Working Group on Legume and Vegetable Viruses (IWGLVV), Ljubljana, Slovenia, 20-23 August, 2008.
Title of Event:	International Conference, Genetic control of plant pathogenic viruses and their vectors: Towards new resistance strategies
Type of Event:	Conference
Location of Event:	El Puerto de Santa Maria, Cadiz, Spain
Date(s) of Event:	23-27 Nov 2008

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