
The Library
Metabolic switches and adaptations deduced from the proteomes of Streptomyces coelicolor wild type and phoP mutant grown in batch culture
Tools
Thomas, L., Hodgson, D. A., Wentzel, A., Nieselt, K., Ellingsen, T. E., Moore, Jonathan D., Morrissey, Edward R., Legaie, Roxane, Wohlleben, W., Rodriguez-Garcia, A., Martin, J. F., Burroughs, Nigel John, Wellington, E. M. H. and Smith, M. C. M. (2012) Metabolic switches and adaptations deduced from the proteomes of Streptomyces coelicolor wild type and phoP mutant grown in batch culture. Molecular & Cellular Proteomics, Vol.11 (No.2). M111.013797. doi:10.1074/mcp.M111.013797 ISSN 1535-9476.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1074/mcp.M111.013797
Abstract
Bacteria in the genus Streptomyces are soil-dwelling oligotrophs and important producers of secondary metabolites. Previously, we showed that global messenger RNA expression was subject to a series of metabolic and regulatory switches during the lifetime of a fermentor batch culture of Streptomyces coelicolor M145. Here we analyze the proteome from eight time points from the same fermentor culture and, because phosphate availability is an important regulator of secondary metabolite production, compare this to the proteome of a similar time course from an S. coelicolor mutant, INB201 (ΔphoP), defective in the control of phosphate utilization. The proteomes provide a detailed view of enzymes involved in central carbon and nitrogen metabolism. Trends in protein expression over the time courses were deduced from a protein abundance index, which also revealed the importance of stress pathway proteins in both cultures. As expected, the ΔphoP mutant was deficient in expression of PhoP-dependent genes, and several putatively compensatory metabolic and regulatory pathways for phosphate scavenging were detected. Notably there is a succession of switches that coordinately induce the production of enzymes for five different secondary metabolite biosynthesis pathways over the course of the batch cultures.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Research Centres > Warwick Systems Biology Centre | ||||
Journal or Publication Title: | Molecular & Cellular Proteomics | ||||
Publisher: | American Society for Biochemistry and Molecular Biology | ||||
ISSN: | 1535-9476 | ||||
Official Date: | 1 February 2012 | ||||
Dates: |
|
||||
Volume: | Vol.11 | ||||
Number: | No.2 | ||||
Page Range: | M111.013797 | ||||
DOI: | 10.1074/mcp.M111.013797 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
![]() |
View Item |