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Metformin treatment may increase Omentin-1 levels in women with polycystic ovary syndrome
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Tan, Bee K., Adya, Raghu, Farhatullah, S., Chen, J., Lehnert, H. and Randeva, Harpal S. (2010) Metformin treatment may increase Omentin-1 levels in women with polycystic ovary syndrome. Diabetes, Vol.59 (No.12). pp. 3023-3031. doi:10.2337/db10-0124 ISSN 0012-1797.
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Official URL: http://dx.doi.org/10.2337/db10-0124
Abstract
OBJECTIVE Polycystic ovary syndrome (PCOS) is associated with the metabolic syndrome. Decreased omentin-1 levels are associated with obesity and diabetes. To study the effects of metformin treatment on omentin-1 levels in PCOS subjects and effects of omentin-1 on in vitro migration and angiogenesis.
RESEARCH DESIGN AND METHODS Serum omentin-1 was measured by ELISA. Angiogenesis was assessed by studying capillary tube formation in human microvascular endothelial cells (HMEC-1) on growth factor reduced Matrigel. Endothelial cell migration assay was performed in a modified Boyden chamber. Nuclear factor-κB (NF-κB) was studied by stably transfecting HMEC-1 cells with a cis-reporter plasmid containing luciferase reporter gene linked to five repeats of NF-κB binding sites. Akt phosphorylation was assessed by Western blotting.
RESULTS Serum omentin-1 was significantly lower in PCOS women (P < 0.05). After 6 months of metformin treatment, there was a significant increase in serum omentin-1 (P < 0.01). Importantly, changes in hs-CRP were significantly negatively correlated with changes in serum omentin-1 (P = 0.036). In vitro migration and angiogenesis were significantly increased in serum from PCOS women (P < 0.01) compared with matched control subjects; these effects were significantly attenuated by metformin treatment (P < 0.01) plausibly through the regulation of omentin-1 levels via NF-κB and Akt pathways. CRP and VEGF induced in vitro migration, and angiogenesis was significantly decreased by omentin-1.
CONCLUSIONS Increases in omentin-1 levels may play a role but are not sufficient to explain the decreased inflammatory and angiogenic effects of sera from metformin-treated PCOS women.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Diabetes | ||||
| Publisher: | American Diabetes Association | ||||
| ISSN: | 0012-1797 | ||||
| Official Date: | December 2010 | ||||
| Dates: |
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| Volume: | Vol.59 | ||||
| Number: | No.12 | ||||
| Page Range: | pp. 3023-3031 | ||||
| DOI: | 10.2337/db10-0124 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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