Barona-Gomez, Francisco and Hodgson, D. A. (2010) Multicopy proC in Streptomyces coelicolor A3(2) elicits a transient production of prodiginines, while proC deletion does not yield a proline auxotroph. Journal of Molecular Microbiology and Biotechnology, Volume 19 (Number 3). pp. 152-158. doi:10.1159/000321502 ISSN 1464-1801.
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Abstract
The last step of proline biosynthesis is typically catalysed by the enzyme Delta(1)-pyrroline-5-carboxylate reductase, encoded by the proC gene. Complete genome sequencing of Streptomyces coelicolor, a soil-dwelling Gram-positive bacterium that uses proline as a precursor for synthesis of prodiginine, revealed a single copy of this gene. Unexpectedly, disruption of this proC homologue (Sco3337) in S. coelicolor M145 yielded a prototrophic strain, yet the reductase activity of Sco3337 was confirmed by complementation of an Escherichia coli proC mutant. Multicopy proC within different genetic contexts elicited a transient production of prodiginines, which showed differential production kinetics of the two most common forms of this natural product produced by S. coelicolor, i.e. streptorubin B (cyclic) and undecylprodigiosin (linear). The metabolic and evolutionary implications of these observations are discussed. Copyright (C) 2010 S. Karger AG, Basel
Item Type: | Journal Article |
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Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology |
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
Library of Congress Subject Headings (LCSH): | Streptomyces coelicolor, Proline -- Synthesis, Protein C gene |
Journal or Publication Title: | Journal of Molecular Microbiology and Biotechnology |
Publisher: | S. Karger AG |
ISSN: | 1464-1801 |
Official Date: | 2010 |
Dates: | Date Event 2010 Published |
Volume: | Volume 19 |
Number: | Number 3 |
Number of Pages: | 7 |
Page Range: | pp. 152-158 |
DOI: | 10.1159/000321502 |
Status: | Peer Reviewed |
Publication Status: | Published |
Funder: | Consejo Nacional de Ciencia y Tecnología (Mexico) [Mexican Council for Science and Technology] (CONACYT) |
Grant number: | 111558, 82319 |
URI: | https://wrap.warwick.ac.uk/4558/ |
Data sourced from Thomson Reuters' Web of Knowledge
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