The Library
Combinatorial genetic transformation of cereals and the creation of metabolic libraries for the carotenoid pathway
Tools
Farre, Gemma, Naqvi, Shaista, Sanahuja, Georgina, Bai, Chao, Zorrilla-López, Uxue, Rivera, Sol M., Canela, Ramon, Sandman, Gerhard, Twyman, Richard M., Capell, Teresa, Zhu, Changfu and Christou, Paul (2012) Combinatorial genetic transformation of cereals and the creation of metabolic libraries for the carotenoid pathway. Methods in Molecular Biology, Vol.847 . pp. 419-435. doi:10.1007/978-1-61779-558-9_33 ISSN 1064-3745.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1007/978-1-61779-558-9_33
Abstract
Combinatorial nuclear transformation is used to generate populations of transgenic plants containing random selections from a collection of input transgenes. This is a useful approach because it provides the means to test different combinations of genes without the need for separate transformation experiments, allowing the comprehensive analysis of metabolic pathways and other genetic systems requiring the coordinated expression of multiple genes. The principle of combinatorial nuclear transformation is demonstrated in this chapter through protocols developed in our laboratory that allow combinations of genes encoding enzymes in the carotenoid biosynthesis pathway to be introduced into rice and a white-endosperm variety of corn. These allow the accumulation of carotenoids to be screened initially by the colour of the endosperm, which ranges from white through various shades of yellow and orange depending on the types and quantities of carotenoids present. The protocols cover the preparation of DNA-coated metal particles, the transformation of corn and rice plants by particle bombardment, the regeneration of transgenic plants, the extraction of carotenoids from plant tissues, and their analysis by high-performance liquid chromatography.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Methods in Molecular Biology | ||||
Publisher: | Springer | ||||
ISSN: | 1064-3745 | ||||
Book Title: | Transgenic Plants | ||||
Official Date: | 2012 | ||||
Dates: |
|
||||
Volume: | Vol.847 | ||||
Page Range: | pp. 419-435 | ||||
DOI: | 10.1007/978-1-61779-558-9_33 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |