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Design and evaluation of novel primers for the detection of genes encoding diverse enzymes of methylotrophy and autotrophy
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Hung, Wei-Lian, Wade, William G, Chen, Yin, Kelly, Donovan P. and Wood, Ann P (2012) Design and evaluation of novel primers for the detection of genes encoding diverse enzymes of methylotrophy and autotrophy. Polish Journal of Microbiology, Vol.61 (No.1). pp. 11-22. ISSN 1733-1331.
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Abstract
The phylogenetic significance of the diversity of key enzymes of methylotrophic and autotrophic metabolism is discussed. Primers for these key enzymes were designed using gene sequences encoding methanol dehydrogenase (mxaF; using subsets from database sequences for 22 Bacteria), hydroxypyruvate reductase (hpr; 36 sequences), methylamine dehydrogenase (mauA; 12 sequences), methanesulfonate monooxygenase (msmA; four sequences), and the ccbL and cbbM genes of ribulose bisphosphate carboxylase (26 and 23 sequences). These were effective in amplifying the correct gene products for the target genes in reference organisms and in test organisms not previously shown to contain the genes, as well as in some methylotrophic Proteobacteria isolated from the human mouth. The availability of the new primers increases the probability of detecting diverse examples of the genes encoding these key enzymes both in natural populations and in isolated bacterial strains.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Polish Journal of Microbiology | ||||
Publisher: | Polskie Towarzystwo Mikrobiologow | ||||
ISSN: | 1733-1331 | ||||
Official Date: | 2012 | ||||
Dates: |
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Volume: | Vol.61 | ||||
Number: | No.1 | ||||
Page Range: | pp. 11-22 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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