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Mapping and candidate-gene screening of the novel Turnip mosaic virus resistance gene retr02 in Chinese cabbage (Brassica rapa L.)
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Qian, Wei, Zhang, Shujiang, Zhang, Shifan, Li, Fei, Zhang, Hui, Wu, Jian, Wang, Xiaowu, Walsh, John A. and Sun, Rifei (2013) Mapping and candidate-gene screening of the novel Turnip mosaic virus resistance gene retr02 in Chinese cabbage (Brassica rapa L.). Theoretical and Applied Genetics, Volume 126 (Number 1). pp. 179-188. doi:10.1007/s00122-012-1972-x ISSN 0040-5752.
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WRAP_Walsh_0380040-lf-061212-walsh__others_theoretical__applied_genetics_2012.pdf - Accepted Version Download (334Kb) | Preview |
Official URL: http://dx.doi.org/10.1007/s00122-012-1972-x
Abstract
The extreme resistance to Turnip mosaic virus observed in the Chinese cabbage (Brassica rapa) line, BP8407, is monogenic and recessive. Bulked segregant analysis was carried out to identify simple sequence repeat and Indel markers linked to this recessive resistance gene, termed recessive Turnip mosaic virus resistance 02 (retr02). Mapping of PCR-specific Indel markers on 239 individuals of a BP8407 × Ji Zao Chun F 2 population, located this resistance gene to a 0.9-cM interval between two Indel markers (BrID10694 and BrID101309) and in scaffold000060 or scaffold000104 on chromosome A04 of the B. rapa genome. Eleven eukaryotic initiation factor 4E (eIF4E) and 14 eukaryotic initiation factor 4G (eIF4G) genes are predicted in the B. rapa genome. A candidate gene, Bra035393 on scaffold000104, was predicted within the mapped resistance locus. The gene encodes the eIF(iso)4E protein. Bra035393 was sequenced in BP8407 and Ji Zao Chun. A polymorphism (A/G) was found in exon 3 between BP8407 and Ji Zao Chun. This gene was analysed in four resistant and three susceptible lines. A correlation was observed between the amino acid substitution (Gly/Asp) in the eIF(iso)4E protein and resistance/susceptibility. eIF(iso)4E has been shown previously to interact with the TuMV genome-linked protein, VPg.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | S Agriculture > SB Plant culture | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Library of Congress Subject Headings (LCSH): | Turnip mosaic virus, Chinese cabbage -- Disease and pest resistance -- Genetic aspects | ||||
| Journal or Publication Title: | Theoretical and Applied Genetics | ||||
| Publisher: | Springer | ||||
| ISSN: | 0040-5752 | ||||
| Official Date: | January 2013 | ||||
| Dates: |
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| Volume: | Volume 126 | ||||
| Number: | Number 1 | ||||
| Page Range: | pp. 179-188 | ||||
| DOI: | 10.1007/s00122-012-1972-x | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Date of first compliant deposit: | 23 December 2015 | ||||
| Date of first compliant Open Access: | 23 December 2015 | ||||
| Funder: | China. Nong ye bu [Ministry of Agriculture] |
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