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Cell death duringDrosophila melanogasterearly oogenesis is mediated through autophagy
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Nezis, I. P., Lamark, Trond, Velentzas, Athanassios D., Rusten, Tor Erik, Bjørkøy, Geir, Johansen, Terje, Papassideri, Issidora S., Stravopodis, Dimitrios J., Margaritis, Lukas H., Stenmark, H. (Harald) and Brech, Andreas (2009) Cell death duringDrosophila melanogasterearly oogenesis is mediated through autophagy. Autophagy, Vol. 5 (No. 3). pp. 298-303. doi:10.4161/auto.5.3.7454 ISSN 1554-8627.
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Official URL: http://dx.doi.org/10.4161/auto.5.3.7454
Abstract
Autophagy is a physiological and evolutionarily conserved process maintaining homeostatic functions, such as protein degradation and organelle turnover. Accumulating data provide evidence that autophagy also contributes to cell death under certain circumstances, but how this is achieved is not well known. Herein, we report that autophagy occurs during developmentally-induced cell death in the female germline, observed in the germarium and during middle developmental stages of oogenesis in Drosophila melanogaster. Degenerating germline cells exhibit caspase activation, chromatin condensation, DNA fragmentation and punctate staining of mCherry-DrAtg8a, a novel marker for monitoring autophagy in Drosophila. Genetic inhibition of autophagy, by removing atg1 or atg7 function, results in significant reduction of DNA fragmentation, suggesting that autophagy acts genetically upstream of DNA fragmentation in this tissue. This study provides new insights into the mechanisms that regulate cell death in vivo during development.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Autophagy | ||||
Publisher: | Landes Bioscience | ||||
ISSN: | 1554-8627 | ||||
Official Date: | 2009 | ||||
Dates: |
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Volume: | Vol. 5 | ||||
Number: | No. 3 | ||||
Page Range: | pp. 298-303 | ||||
DOI: | 10.4161/auto.5.3.7454 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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