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Virus-induced gene complementation reveals a transcription factor network in modulation of tomato fruit ripening
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Zhou, Tao, Zhang, Hang, Lai, Tongfei, Qin, Cheng, Shi, Nongnong, Wang, Huizhong, Jin, Mingfei, Zhong, Silin, Fan, Zaifeng, Liu, Yule, Wu, Zirong, Jackson, Stephen D. , Giovannoni, James J., Rolin, Dominique, Gallusci, Philippe and Hong, Yiguo (2012) Virus-induced gene complementation reveals a transcription factor network in modulation of tomato fruit ripening. Scientific Reports, Vol.2 . p. 836. doi:10.1038/srep00836 ISSN 2045-2322.
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Official URL: http://dx.doi.org/10.1038/srep00836
Abstract
Plant virus technology, in particular virus-induced gene silencing, is a widely used reverse- and forward-genetics tool in plant functional genomics. However the potential of virus technology to express genes to induce phenotypes or to complement mutants in order to understand the function of plant genes is not well documented. Here we exploit Potato virus X as a tool for virus-induced gene complementation (VIGC). Using VIGC in tomato, we demonstrated that ectopic viral expression of LeMADS-RIN, which encodes a MADS-box transcription factor (TF), resulted in functional complementation of the non-ripening rin mutant phenotype and caused fruits to ripen. Comparative gene expression analysis indicated that LeMADS-RIN up-regulated expression of the SBP-box (SQUAMOSA promoter binding protein-like) gene LeSPL-CNR, but down-regulated the expression of LeHB-1, an HD-Zip homeobox TF gene. Our data support the hypothesis that a transcriptional network may exist among key TFs in the modulation of fruit ripening in tomato.
Item Type: | Journal Article | ||||
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Subjects: | S Agriculture > SB Plant culture | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Potato virus X, Complementation (Genetics), Tomatoes -- Genetics, Fruit -- Ripening -- Genetics | ||||
Journal or Publication Title: | Scientific Reports | ||||
Publisher: | Nature Publishing Group | ||||
ISSN: | 2045-2322 | ||||
Official Date: | 2012 | ||||
Dates: |
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Volume: | Vol.2 | ||||
Page Range: | p. 836 | ||||
DOI: | 10.1038/srep00836 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 23 December 2015 | ||||
Date of first compliant Open Access: | 23 December 2015 | ||||
Funder: | Great Britain, China, Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Royal Society (Great Britain), Hangzhou shi fan da xue | ||||
Grant number: | BBS/E/H/00YH0271 (BBSRC), 2007/R1/Hong (RS), 2006R2/China/KCW/Hong (RS) | ||||
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