The Library
Comprehensive detection of phototrophic sulfur bacteria using PCR primers that target reverse dissimilatory sulfite reductase gene
Tools
Tools
Tools
Mori, Yumi, Purdy, Kevin J., Oakley, Brian B. and Kondo, Ryuji (2010) Comprehensive detection of phototrophic sulfur bacteria using PCR primers that target reverse dissimilatory sulfite reductase gene. Microbes and Environments, Vol.25 (No.3). pp. 190-196. doi:10.1264/jsme2.ME10109 ISSN 1342-6311.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1264/jsme2.ME10109
Abstract
A new set of primers for the detection of phototrophic sulfur bacteria in natural environments is described. The primers target the alpha-subunit of the reverse dissimilatory sulfite reductase gene (dsrA). PCR-amplification resulted in products of the expected size from all the phototrophic strains tested, including purple sulfur and green sulfur bacteria. Seventy-nine clones obtained from environmental DNA using the primers were sequenced and all found to be closely related to the dsrA of purple sulfur bacteria and green sulfur bacteria. This newly developed PCR assay targeting dsrA is rapid and simple for the detection of phototrophic sulfur bacteria in situ and superior to the use of culture-dependent techniques.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology |
||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Microbes and Environments | ||||
| Publisher: | Japanese Soc Microbial Ecology, Dept Bioresource Science [Nihon Biseibutsu Seitai Gakkai] | ||||
| ISSN: | 1342-6311 | ||||
| Official Date: | 3 September 2010 | ||||
| Dates: |
|
||||
| Volume: | Vol.25 | ||||
| Number: | No.3 | ||||
| Number of Pages: | 7 | ||||
| Page Range: | pp. 190-196 | ||||
| DOI: | 10.1264/jsme2.ME10109 | ||||
| Status: | Not Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Japan Society for the Promotion of Science, Fukui Prefectural Fund for the Promotion of Science, Kurita Water and Environment Foundation, EU Commission | ||||
| Grant number: | MEXT-CT-2005-024112 |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
