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Waking up streptomyces secondary metabolism by constitutive expression of activators or genetic disruption of repressors
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Aigle, Bertrand and Corre, Christophe (2012) Waking up streptomyces secondary metabolism by constitutive expression of activators or genetic disruption of repressors. Methods in Enzymology, Vol.517 . pp. 343-366. doi:10.1016/B978-0-12-404634-4.00017-6 ISSN 0076-6879.
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Official URL: http://dx.doi.org/10.1016/B978-0-12-404634-4.00017...
Abstract
Streptomycete bacteria are renowned as a prolific source of natural products with diverse biological activities. Production of these metabolites is often subject to transcriptional regulation: the biosynthetic genes remain silent until the required environmental and/or physiological signals occur.
Consequently, in the laboratory environment, many gene clusters that direct the biosynthesis of natural products with clinical potential are not expressed or at very low level preventing the production/detection of the associated metabolite. Genetic engineering of streptomycetes can unleash the production of many new natural products. This chapter describes the overexpression of pathway-specific activators, the genetic disruption of pathway-specific repressors, and the main strategy used to identify and characterize new natural products from these engineered Streptomyces strains.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Journal or Publication Title: | Methods in Enzymology | ||||
Publisher: | Academic Press | ||||
ISSN: | 0076-6879 | ||||
Book Title: | Natural Product Biosynthesis by Microorganisms and Plants, Part C | ||||
Official Date: | October 2012 | ||||
Dates: |
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Volume: | Vol.517 | ||||
Page Range: | pp. 343-366 | ||||
DOI: | 10.1016/B978-0-12-404634-4.00017-6 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Description: | Chapter 17 of "Natural Product Biosynthesis by Microorganisms and Plants, Part C" edited by David A. Hopwood, ISBN: 9780124046344 |
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