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Dimerization and DNA-dependent aggregation of the Escherichia coli nucleoid protein and chaperone CbpA
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Cosgriff, Sarah, Chintakayala, Kiran, Chim, Ya Tsz A., Chen, Xinyong, Allen, Stephanie (Researcher in Pharmacy), Lovering, Andrew L. and Grainger, David C. (2010) Dimerization and DNA-dependent aggregation of the Escherichia coli nucleoid protein and chaperone CbpA. Molecular Microbiology, Volume 77 (Number 5). pp. 1289-1300. doi:10.1111/j.1365-2958.2010.07292.x ISSN 0950-382X.
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WRAP_Grainger_j.1365-2958.2010.07292 - Published Version Download (1772Kb) |
Official URL: http://dx.doi.org/10.1111/j.1365-2958.2010.07292.x
Abstract
The Escherichia coli curved DNA-binding protein A (CbpA) is a nucleoid-associated DNA-binding factor and chaperone that is expressed at high levels as cells enter stationary phase. Using a combination of genetics, biochemistry, structural modelling and single-molecule atomic force microscopy we have examined dimerization of, and DNA binding by, CbpA. Our data show that CbpA dimerization is driven by a hydrophobic surface comprising amino acid side chains W287 and L290 located on the same side of an alpha helix close to the C-terminus of CbpA. Derivatives of CbpA that are unable to dimerize are also unable to bind DNA. Free in solution, CbpA can exist as either a monomer or dimer. However, when bound to DNA, CbpA forms large aggregates that can protect DNA from degradation by nucleases. These CbpA-DNA aggregates are similar in morphology to protein-DNA complexes formed by the DNA-binding protein from starved cells (Dps), the only other stationary phase-specific nucleoid protein. Conversely, protein-DNA complexes formed by Fis, the major growth phase nucleoid protein, have a markedly different appearance.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
Library of Congress Subject Headings (LCSH): | Escherichia coli, Escherichia coli -- Genetics , DNA-binding proteins | ||||
Journal or Publication Title: | Molecular Microbiology | ||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
ISSN: | 0950-382X | ||||
Official Date: | September 2010 | ||||
Dates: |
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Volume: | Volume 77 | ||||
Number: | Number 5 | ||||
Number of Pages: | 12 | ||||
Page Range: | pp. 1289-1300 | ||||
DOI: | 10.1111/j.1365-2958.2010.07292.x | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 3 December 2015 | ||||
Date of first compliant Open Access: | 3 December 2015 | ||||
Funder: | Wellcome Trust (London, England), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||
Grant number: | BB/H010289/1 (BBSRC) |
Data sourced from Thomson Reuters' Web of Knowledge
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