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Protein production in Saccharomyces cerevisiae for systems biology studies
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Malys, Naglis, Wishart, Jill A., Oliver, Stephen G. and McCarthy, John E. G. (2011) Protein production in Saccharomyces cerevisiae for systems biology studies. In: Jameson, Daniel and Verma , Malkhey and Westerhoff, Hans V. , (eds.) Methods in Systems Biology. Methods in Enzymology, Vol.500 . Academic Press, pp. 197-212. ISBN 9780123851185
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Official URL: http://dx.doi.org/10.1016/B978-0-12-385118-5.00011...
Abstract
Proteins together with metabolites, nucleic acids, lipids, and other intracellular molecules form biological systems that involve networks of functional and physical interactions. To understand these interactions and the many other characteristics of proteins in the context of biochemical networks and systems biology, research aimed at studying medium and large sets of proteins is required. This either involves an investigation focused on individual protein activities in the mixture (e.g., cell extracts) or a protein characterization in the isolated form. This chapter provides an overview on the currently available resources and strategies for isolation of proteins from Saccharomyces cerevisiae. The use of standardized gene expression systems is discussed, and protein production protocols applied to the data generation pipeline for systems biology are described in detail.
Item Type: | Book Item | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Series Name: | Methods in Enzymology | ||||
Journal or Publication Title: | Methods in Enzymology | ||||
Publisher: | Academic Press | ||||
ISBN: | 9780123851185 | ||||
ISSN: | 0076-6879 | ||||
Book Title: | Methods in Systems Biology | ||||
Editor: | Jameson, Daniel and Verma , Malkhey and Westerhoff, Hans V. | ||||
Official Date: | 2011 | ||||
Dates: |
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Volume: | Vol.500 | ||||
Number of Pages: | 715 | ||||
Page Range: | pp. 197-212 | ||||
DOI: | 10.1016/B978-0-12-385118-5.00011-6 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published |
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