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Calculations of flow-induced orientation distributions for analysis of linear dichroism spectroscopy
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McLachlan, James R. A., Smith, D. J. (David J.), Chmel, Nikola Paul and Rodger, Alison (2013) Calculations of flow-induced orientation distributions for analysis of linear dichroism spectroscopy. Soft Matter, Volume 9 (Issue 20). 4977 -4984 . doi:10.1039/c3sm27419e ISSN 1744-683X.
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Official URL: http://pubs.rsc.org/en/Journals/JournalIssues/SM
Abstract
Spectroscopic techniques involving flow-oriented samples and polarised light, such as linear dichroism (LD), are becoming in- creasingly useful to probe biomacromolecular assemblies. However, the magnitude of the signal and in some cases the shape of the spectrum are dependent on the distribution of the orientations of the molecules in the sample. Despite great progress in the modelling of dilute and semi-dilute suspension mechanics, these theories have had remarkably little impact on the community practising LD. We perform calculations with a model combining Brownian effects and rotations in a steady shear flow of a dilute suspension of rigid, rodlike particles. We calculate the time-dependent probability density functions for the orientation distri- butions of three biomolecular assemblies: M13 bacteriophage, DNA molecules of well-defined length, and FtsZ protofilaments. Our calculations allow us to compute directly (rather than infer from experiment) the LD orientation parameter, S, for such assemblies. The results from the model are consistent with experiment for M13 bacteriophage and allow us to estimate S empir- ically for reasonably short DNA molecules. In analysing the particle size distribution for the process of FtsZ polymerisation, we find that results from the model aid our understanding of the process.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Library of Congress Subject Headings (LCSH): | Linear dichroism, Spectrum analysis, Polarization spectroscopy, Shear flow | ||||
Journal or Publication Title: | Soft Matter | ||||
Publisher: | Royal Society of Chemistry | ||||
ISSN: | 1744-683X | ||||
Official Date: | 2013 | ||||
Dates: |
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Volume: | Volume 9 | ||||
Number: | Issue 20 | ||||
Page Range: | 4977 -4984 | ||||
DOI: | 10.1039/c3sm27419e | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 24 December 2015 | ||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Science City Research Alliance | ||||
Grant number: | EP/F500378/1 (EPSRC), BB/F011199/1 (BBSRC) |
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