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Xanthomonas campestris pv. campestris race 1 is the main causal agent of black rot of Brassicas in Southern Mozambique
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Bila, J., Mortensen, C. N., Andresen, M., Vicente, Joana G. and Wulff, E. G. (2013) Xanthomonas campestris pv. campestris race 1 is the main causal agent of black rot of Brassicas in Southern Mozambique. African Journal of Biotechnology, Volume 12 (Number 6). pp. 602-610. ISSN 1684-5315.
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Official URL: http://dx.doi.org/10.5897/AJB12.2455
Abstract
Severe outbreaks of bacterial black rot caused by Xanthomonas campestris pv. campestris (Xcc) were observed in Brassica production fields of Southern Mozambique. The causal agent of the disease in the Mahotas and Chòkwé districts was identified and characterised. In total, 83 Xanthomonas-like strains were isolated from seed samples and leaves of cabbage and tronchuda cole with typical symptoms of the disease. Forty-six out of the 83 strains were found to be putative Xcc in at least one of the tests used: Classical biochemical assays, enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies, Biolog identification system, polymerase chain reaction (PCR) with specific primers and pathogenicity tests. The ELISA tests were positive for 43 strains. Biolog identified 43 strains as Xanthomonas, but only 32 as Xcc. PCR tests with primers targeting a fragment of the hrpF gene were positive for all 46 strains tested. Three strains were not pathogenic or weakly pathogenic and all other strains caused typical black rot symptoms in brassicas. Race type differentiation tests revealed the Xcc strains from Mozambique as members of race 1. The prevalence of this pathogenic race of the Xcc pathogen in Mozambique should be considered when black rot resistant cultivars are evaluated or introduced into the production regions of this country.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QK Botany |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Crucifer black rot -- Mozambique, Cruciferae -- Diseases and pests -- Mozambique | ||||
Journal or Publication Title: | African Journal of Biotechnology | ||||
Publisher: | Academic Journals | ||||
ISSN: | 1684-5315 | ||||
Official Date: | February 2013 | ||||
Dates: |
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Volume: | Volume 12 | ||||
Number: | Number 6 | ||||
Page Range: | pp. 602-610 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 1 August 2016 | ||||
Date of first compliant Open Access: | 1 August 2016 | ||||
Funder: | DANIDA, ENRECA (DANIDA) | ||||
Grant number: | ENRECA Project Life - 731 | ||||
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