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Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine
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Gladstone, Rebecca A., Loman, Nicholas J., Constantinidou, Chrystala, Tocheva, Anna S., Jefferies, Johanna M. C., Faust, Saul N., O’Connor, Leigh, Chan, Jacqueline, Pallen, Mark J. and Clarke, Stuart C. (2013) Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine. PLoS One, Volume 8 (Number 5). e64731. doi:10.1371/journal.pone.0064731 ISSN 1932-6203.
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WRAP_Gladstone_journal.pone.0064731.pdf - Published Version Available under License Creative Commons Attribution. Download (553Kb) | Preview |
Official URL: http://dx.doi.org/10.1371/journal.pone.0064731
Abstract
Streptococcus pneumoniae causes invasive infections, primarily at the extremes of life. A seven-valent conjugate vaccine (PCV7) is used to protect against invasive pneumococcal disease in children. Within three years of PCV7 introduction, we observed a fourfold increase in serotype 6C carriage, predominantly due to a single clone. We determined the whole-genome sequences of nineteen S. pneumoniae serotype 6C isolates, from both carriage (n = 15) and disease (n = 4) states, to investigate the emergence of serotype 6C in our population, focusing on a single multi-locus sequence type (MLST) clonal complex 395 (CC395). A phylogenetic network was constructed to identify different lineages, followed by analysis of variability in gene sets and sequences. Serotype 6C isolates from this single geographical site fell into four broad phylogenetically distinct lineages. Variation was seen in the 6C capsular locus and in sequences of genes encoding surface proteins. The largest clonal complex was characterised by the presence of lantibiotic synthesis locus. In our population, the 6C capsular locus has been introduced into multiple lineages by independent capsular switching events. However, rapid clonal expansion has occurred within a single MLST clonal complex. Worryingly, plasticity exists within current and potential vaccine-associated loci, a consideration for future vaccine use, target selection and design.
| Item Type: | Journal Article | ||||||
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| Subjects: | Q Science > QR Microbiology > QR180 Immunology R Medicine > R Medicine (General) R Medicine > RA Public aspects of medicine |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Streptococcus pneumoniae -- Vaccination -- Complications, Streptococcus pneumoniae -- Effect of drugs on, Vaccination, Immunization, Diagnostic microbiology, Serology, Genomics, Molecular genetics | ||||||
| Journal or Publication Title: | PLoS One | ||||||
| Publisher: | Public Library of Science | ||||||
| ISSN: | 1932-6203 | ||||||
| Official Date: | 28 May 2013 | ||||||
| Dates: |
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| Volume: | Volume 8 | ||||||
| Number: | Number 5 | ||||||
| Article Number: | e64731 | ||||||
| DOI: | 10.1371/journal.pone.0064731 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||
| Date of first compliant deposit: | 24 December 2015 | ||||||
| Date of first compliant Open Access: | 24 December 2015 | ||||||
| Funder: | BUPA Foundation, Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||
| Grant number: | BB/E011179/1 (BBSRC) |
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