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Conjugation-induced fluorescent labeling of proteins and polymers using dithiomaleimides
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Robin, Mathew P., Wilson, Paul, Mabire, Anne B., Kiviaho, Jenny K., Raymond, Jeffery E., Haddleton, David M. and O’Reilly, Rachel K. (2013) Conjugation-induced fluorescent labeling of proteins and polymers using dithiomaleimides. Journal of the American Chemical Society, Volume 135 (Number 8). pp. 2875-2878. doi:10.1021/ja3105494 ISSN 0002-7863.
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Official URL: http://dx.doi.org/10.1021/ja3105494
Abstract
Dithiomaleimides (DTMs) with alkyl substituents are shown to be a novel class of highly emissive fluorophores. Variable solubility and further functionalization can easily be tailored through the choice of N and S substituents. Inclusion of a DTM unit into a ROP/RAFT initiator or insertion into the disulfide bond of salmon calcitonin (sCT) demonstrates the utility for fluorescent labeling of polymers and proteins. Simultaneous PEGylation and fluorescent labeling of sCT is also demonstrated, using the DTM unit as both a linker and a fluorophore. It is anticipated that DTMs will offer an attractive alternative to commonly used bulky, planar fluorophores.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Library of Congress Subject Headings (LCSH): | Chemicals, Fluorescent polymers, Polymers, Macromolecules, Fluorescence spectroscopy | ||||
Journal or Publication Title: | Journal of the American Chemical Society | ||||
Publisher: | American Chemical Society | ||||
ISSN: | 0002-7863 | ||||
Official Date: | 6 February 2013 | ||||
Dates: |
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Volume: | Volume 135 | ||||
Number: | Number 8 | ||||
Page Range: | pp. 2875-2878 | ||||
DOI: | 10.1021/ja3105494 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 24 December 2015 | ||||
Date of first compliant Open Access: | 24 December 2015 | ||||
Funder: | Advantage West Midlands (AWM), Science City Research Alliance, European Regional Development Fund (ERDF) |
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