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Hsc70-induced changes in clathrin-auxilin cage structure suggest a role for clathrin light chains in cage disassembly
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Young, Anna, Stoilova-McPhie, Svetla, Rothnie, Alice, Vallis, Yvonne, Harvey-Smith, Phillip, Ranson, Neil, Kent, Helen, Brodsky, Frances M., Pearse, Barbara M. F., Roseman, Alan and Smith, Corinne J. (2013) Hsc70-induced changes in clathrin-auxilin cage structure suggest a role for clathrin light chains in cage disassembly. Traffic, Volume 14 (Number 9). pp. 987-996. doi:10.1111/tra.12085 ISSN 1398-9219.
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Official URL: http://dx.doi.org/10.1111/tra.12085
Abstract
The molecular chaperone, Hsc70, together with its co-factor, auxilin, facilitates the ATP-dependent removal of clathrin during clathrin-mediated endocytosis in cells. We have used cryo-electron microscopy to determine the 3D structure of a complex of clathrin, auxilin401-910 and Hsc70 at pH 6 in the presence of ATP, frozen within 20 seconds of adding Hsc70 in order to visualize events that follow the binding of Hsc70 to clathrin and auxilin before clathrin disassembly. In this map, we observe density beneath the vertex of the cage that we attribute to bound Hsc70. This density emerges asymmetrically from the clathrin vertex, suggesting preferential binding by Hsc70 for one of the three possible sites at the vertex. Statistical comparison with a map of whole auxilin and clathrin previously published by us reveals the location of statistically significant differences which implicate involvement of clathrin light chains in structural rearrangements which occur after Hsc70 is recruited. Clathrin disassembly assays using light scattering suggest that loss of clathrin light chains reduces the efficiency with which auxilin facilitates this reaction. These data support a regulatory role for clathrin light chains in clathrin disassembly in addition to their established role in regulating clathrin assembly.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Endocytosis, Cell physiology, Cytology, Molecular biology -- Research | ||||
Journal or Publication Title: | Traffic | ||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
ISSN: | 1398-9219 | ||||
Official Date: | September 2013 | ||||
Dates: |
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Volume: | Volume 14 | ||||
Number: | Number 9 | ||||
Page Range: | pp. 987-996 | ||||
DOI: | 10.1111/tra.12085 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 25 December 2015 | ||||
Date of first compliant Open Access: | 25 December 2015 | ||||
Funder: | Royal Society (Great Britain), Medical Research Council (Great Britain) (MRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), National Institutes of Health (U.S.) (NIH), Wellcome Trust (London, England) | ||||
Grant number: | G120/488 (MRC), G060/125 (MRC) ; GM038093, P41 RR001081 (NIH) ; 055663/Z/98/2 (WT) |
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