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Age-matched dendritic cell subpopulations reference values in childhood
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Heinze, A., Elze, M. C., Kloess, S., Ciocarlie, O., Königs, C., Betz, S., Bremm, M., Esser, R., Klingebiel, T., Serban, M., Hutton, Jane and Koehl, U. (2013) Age-matched dendritic cell subpopulations reference values in childhood. Scandinavian Journal of Immunology, Volume 77 (Number 3). pp. 213-220. doi:10.1111/sji.12024 ISSN 0300-9475.
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Official URL: http://dx.doi.org/10.1111/sji.12024
Abstract
Dendritic cells (DCs) are the most potent antigen-presenting cells and are the key link between the innate and adaptive immune response. Only a few reports with study populations of up to 50 individuals have been published with age-based reference values for DC subpopulations in healthy children. Therefore, we aimed to establish reference ranges in a larger study population of 100 healthy children, which allowed age-matched subgroups. Most previous studies were performed using a dual-platform approach. In this study, a single-platform approach in a lyse no-wash procedure was used. DC subpopulations were defined as follows: CD45+CD85k+HLA-DR+CD14−CD16−CD33+ cells as myeloid DCs (mDCs) and CD45+CD85k+HLA-DR+CD14−CD16−CD123+ cells as plasmacytoid DCs (pDCs). Reference ranges were established using a semi-parametric regression of age-matched absolute and relative DC counts. We found a significant decline with increasing age in the medians of mDCs (P = 0.0003) and pDCs per μl peripheral blood (PB) (P = 0.004) and in the 50%, 90% and 95% reference ranges. We also identified significantly lower absolute cell counts of mDCs per μl PB in girls than in boys for all age groups (P = 0.0015). Due to the larger paediatric study population and single-platform approach, this study may give a more precise overview of the normal age-matched development of DC subpopulations and may provide a basis for analyzing abnormal DC counts in different illnesses or therapies such as post stem cell transplantation.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Statistics | ||||
Journal or Publication Title: | Scandinavian Journal of Immunology | ||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
ISSN: | 0300-9475 | ||||
Official Date: | 2013 | ||||
Dates: |
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Volume: | Volume 77 | ||||
Number: | Number 3 | ||||
Page Range: | pp. 213-220 | ||||
DOI: | 10.1111/sji.12024 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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