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A role for sigma factor σE in corynebacterium pseudotuberculosis resistance to nitric oxide/peroxide stress
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Pacheco, Luis G. C., Castro, Thiago L. P., Carvalho, Rodrigo D. O., Moraes, Pablo M., Dorella, Fernanda A., Carvalho, Natália B., Slade, Susan E., Scrivens, James H., Feelisch, Martin, Meyer, Roberto, Miyoshi, Anderson, Oliveira, Sérgio C., Dowson, Christopher G. and Azevedo, Vasco (2012) A role for sigma factor σE in corynebacterium pseudotuberculosis resistance to nitric oxide/peroxide stress. Frontiers in Microbiology, Volume 3 . Article number 126. doi:10.3389/fmicb.2012.00126 ISSN 1664-302X.
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Official URL: http://dx.doi.org/10.3389/fmicb.2012.00126
Abstract
Pathogenic intracellular bacteria can respond to antimicrobial mechanisms of the host cell through transient activation of stress-responsive genes by alternative sigma (σ) factors of the RNA polymerase. We evaluated the contribution of the extracytoplasmic function sigma factor σE for Corynebacterium pseudotuberculosis resistance to stress conditions resembling those found intracellularly during infection. A sigE-null mutant strain (ΔsigE) of this bacterium was more susceptible in vitro to acidic pH, cell surface stressors, and biologically relevant concentrations of nitric oxide (NO). The same mutant strain was unable to persist in C57BL/6 mice but remained infective in mice lacking inducible nitric oxide synthase (iNOS), confirming the significance of σE for resistance to nitric oxide/peroxide stress in vivo. High-throughput proteomic analysis identified NO-responsive extracellular proteins of C. pseudotuberculosis and demonstrated the participation of σE in composition of this bacterium’s exoproteome.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Frontiers in Microbiology | ||||
Publisher: | Frontiers Research Foundation | ||||
ISSN: | 1664-302X | ||||
Official Date: | 2012 | ||||
Dates: |
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Volume: | Volume 3 | ||||
Page Range: | Article number 126 | ||||
DOI: | 10.3389/fmicb.2012.00126 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published |
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