Mutopo, David S. (1980) Studies on the binding of bilirubin to albumin. PhD thesis, University of Warwick.

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Abstract

In this thesis, the
work undertaken
in
an attempt to gain
insight into the location of the binding
site of
bilirubin is
described.
The history, properties and
biosynthesis
of
both bilirubin and
albumin are reviewed
in Chapter One. Chapter Two
contains an account
of the novel one-step method of
labelling bilirubin
of
high
yield
and specific activity.
In Chapter Three, the effect of
light
on
bilirubin in the presence and absence of albumin,
followed by the
labelling
of the albumin
in
an attempt to obtain
information
regarding
the nature of the amino acid residues
involved in the binding
of
bilirubin to albumin.
Attachment
of
labelled bilirubin to albumin
enabled the possible site on the latter to be followed during degrading
procedures.
Possible
effect of this photo-induced
binding
on
phototherapy
is described.
Chapter Four
contains an account of
the synthesis and binding
studies of mesobilirubin.
Fluorescence
and spectroscopic methods
are employed to determine the association constants of mesobilirubin
and comparison with
bilirubin made.
The importance
of the vinyl
groups
in bilirubin binding is
cited and the possibility of mesobilirubin
being
a
kernicteric
pigment
is hypothesized.

Item Type:	Thesis (PhD)
Subjects:	Q Science > QD Chemistry
Library of Congress Subject Headings (LCSH):	Bilirubin, Albumins, Binding sites (Biochemistry)
Official Date:	1980
Dates:	Date Event 1980 Submitted
Institution:	University of Warwick
Theses Department:	Department of Chemistry
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Hutchinson, D. W. (David Wesley)
Sponsors:	United Nations
Extent:	vii, [102] leaves : illustrations
Language:	eng

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