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bZIP67 regulates the omega-3 fatty acid content of arabidopsis seed oil by activating FATTY ACID DESATURASE3
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Mendes, Ana, Kelly, Amélie A., van Erp, Harrie, Shaw, Eve, Powers, Stephen J., Kurup, Smita and Eastmond, Peter J. (2013) bZIP67 regulates the omega-3 fatty acid content of arabidopsis seed oil by activating FATTY ACID DESATURASE3. Plant Cell, Volume 25 (Number 8). pp. 3104-3116. doi:10.1105/tpc.113.116343 ISSN 1040-4651.
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Official URL: http://dx.doi.org/10.1105/tpc.113.116343
Abstract
Arabidopsis thaliana seed maturation is accompanied by the deposition of storage oil, rich in the essential ω-3 polyunsaturated fatty acid α-linolenic acid (ALA). The synthesis of ALA is highly responsive to the level of FATTY ACID DESATURASE3 (FAD3) expression, which is strongly upregulated during embryogenesis. By screening mutants in LEAFY COTYLEDON1 (LEC1)–inducible transcription factors using fatty acid profiling, we identified two mutants (lec1-like and bzip67) with a seed lipid phenotype. Both mutants share a substantial reduction in seed ALA content. Using a combination of in vivo and in vitro assays, we show that bZIP67 binds G-boxes in the FAD3 promoter and enhances FAD3 expression but that activation is conditional on bZIP67 association with LEC1-LIKE (L1L) and NUCLEAR FACTOR-YC2 (NF-YC2). Although FUSCA3 and ABSCISIC ACID INSENSITIVE3 are required for L1L and bZIP67 expression, neither protein is necessary for [bZIP67:L1L:NF-YC2] to activate FAD3. We conclude that a transcriptional complex containing L1L, NF-YC2, and bZIP67 is induced by LEC1 during embryogenesis and specifies high levels of ALA production for storage oil by activating FAD3 expression
Item Type: | Journal Article | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Journal or Publication Title: | Plant Cell | ||||||||
Publisher: | American Society of Plant Biologists | ||||||||
ISSN: | 1040-4651 | ||||||||
Official Date: | 30 August 2013 | ||||||||
Dates: |
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Volume: | Volume 25 | ||||||||
Number: | Number 8 | ||||||||
Page Range: | pp. 3104-3116 | ||||||||
DOI: | 10.1105/tpc.113.116343 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) |
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