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Characterisation of fusarium pathogens in the UK
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Vágány, Viktória (2012) Characterisation of fusarium pathogens in the UK. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b2680876~S1
Abstract
The primary aim of this project was to identify and characterise Fusarium
species associated with the basal rot of Allium species and internal fruit rot of sweet
peppers in the UK. The secondary objective was to develop quick molecular markers
to identify Fusarium oxysporum f. sp. cepae (FOC) causing onion basal rot. Isolates
representing diverse Fusarium species taken from onions, garlic, shallot and leeks
obtained from different production and processing sites in the UK were collected. F.
proliferatum was found for the first time to be a causal agent of onion basal rot in the
UK, but F. oxysporum was by far the most common species and F. oxysporum
isolates belonged to at least two different genotypes based on a sequence comparison
of several “housekeeping” genes, and overall, appeared to be polyphyletic. None of
the housekeeping genes studied correlate with pathogenicity. Secreted in xylem (SIX)
genes offer more promise for the specific identification of F. oxysporum formae
speciales (Lievens et al., 2009a) and a homologue of the SIX7 gene was found only
in a few FOC isolates suggesting that SIX7 is not absolutely necessary for
pathogenicity. Whole genome sequencing of a FOC isolate was carried out in order
to understand pathogenicity and identify novel effector genes. This work revealed the
presence of further homologues of published SIX genes, namely SIX3, SIX5 and
SIX9. The presence of SIX3 and SIX5 has only been reported from F. oxysporum f.
sp. lycopersici previously. Additionally, screening of eleven new candidate effector
genes suggested that FOC isolates have different gene sets which correspond to the
continuous variation of aggressiveness found within the FOC population. Fusarium
lactis, F. proliferatum and F. solani were identified in association with internal fruit
rot of sweet pepper obtained from three different production sites in the UK.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QK Botany S Agriculture > SB Plant culture |
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Library of Congress Subject Headings (LCSH): | Fusarium diseases of plants -- Great Britain, Phytopathogenic microorganisms, Plant-pathogen relationships | ||||
Official Date: | September 2012 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | School of Life Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Barbara, Dez J.; Sreenivasaprasad, Surapareddy | ||||
Extent: | xvii, 278 leaves. | ||||
Language: | eng |
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