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High-resolution1H NMR investigations of the oxidative consumption of salivary biomolecules by oral rinse peroxides
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Chang, Hubert, Silwood, Christopher J. L., Lynch, Edward, Dr. and Grootveld, Martin (2013) High-resolution1H NMR investigations of the oxidative consumption of salivary biomolecules by oral rinse peroxides. Acta Odontologica Scandinavica, Volume 71 (Number 1). pp. 223-235. doi:10.3109/00016357.2012.658082 ISSN 0001-6357.
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Official URL: http://dx.doi.org/10.3109/00016357.2012.658082
Abstract
Background: A multicomponent evaluation of the oxidative consumption of salivary biomolecules by a tooth-whitening oral rinse preparation has been performed using high-resolution proton (1H) nuclear magnetic resonance spectroscopy (NMR). Methods. Unstimulated human saliva samples (n = 12) were treated with aliquots of the oral rinse tested and 600 MHz 1H NMR spectra acquired on these samples demonstrated that hydrogen peroxide (H2O2) and/or peroxodisulphate (S2O8 2–) present in this product gave rise to the oxidative decarboxylation of the salivary electron-donor pyruvate (to acetate and CO2), and also oxidized methionine (a precursor to volatile sulphur compounds responsible for oral malodour), and malodourous trimethylamine to methionine sulphoxide and trimethylamine-N-oxide, respectively (reductions observed in the salivary concentrations of each biomolecular peroxide-scavenging agent were all extremely statistically significant, p < 0.005). Results. Experiments conducted on chemical model systems confirmed the consumption of pyruvate by this product, and also revealed that the amino acids cysteine and methionine were oxidatively transformed to cystine and methionine sulphoxide, respectively. Conclusions. High-field 1H NMR analysis provides much valuable molecular information regarding the fate of tooth-whitening oxidants in human saliva and permits an assessment of the mechanisms of action of oral healthcare products containing these agents. The biochemical and potential therapeutic significance of the results obtained are discussed.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Social Sciences > Warwick Business School Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Acta Odontologica Scandinavica | ||||
| Publisher: | Informa Healthcare | ||||
| ISSN: | 0001-6357 | ||||
| Official Date: | January 2013 | ||||
| Dates: |
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| Volume: | Volume 71 | ||||
| Number: | Number 1 | ||||
| Page Range: | pp. 223-235 | ||||
| DOI: | 10.3109/00016357.2012.658082 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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