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Experimental evolution of herbicide resistance in chlamydomonas reinhardtii
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Lagator, Mato (2012) Experimental evolution of herbicide resistance in chlamydomonas reinhardtii. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b2683241~S1
Abstract
Our
understanding
of
the
evolutionary
dynamics
of
selection
for
herbicide
resistance
is
limited
by
the
time
and
space
required
to
conduct
meaningful
selection
experiments
in
higher
plants.
This
constrains
the
study
of
the
dynamics
of
resistance
evolution
predominantly
to
mathematical
models.
The
primary
goal
of
this
thesis
was
to
overcome
these
limitations,
and
to
study
the
evolutionary
phenomena
underpinning
several
management
strategies.
To
do
so,
a
series
of
experimental
evolution
studies
were
conducted
using
Chlamydomonas
reinhardtii,
a
single-‐cell
green
chlorophyte
susceptible
to
a
range
of
commercial
herbicides.
In
particular,
this
thesis
explored
the
impact
of
herbicide
sequences,
rotations
and
mixtures,
as
well
the
impact
of
herbicide
dose,
on
evolution
of
resistance.
Applying
herbicides
in
sequence
allowed
the
study
of
the
impact
of
environmental
perturbation
on
the
dynamics
of
resistance
and
the
associated
fitness
costs,
finding
more
rapid
selection
for
resistance
to
a
second
and
third
mode
of
action
in
some
populations.
Cycling
between
herbicides
creates
conditions
of
temporal
environmental
heterogeneity,
the
outcomes
of
which
are
not
easily
predictable
as
resistance
was
slowed
down
in
some
cycling
regimes,
while
in
others
it
accelerated
the
evolution
of
resistance
or
gave
rise
to
cross-‐resistance.
Herbicide
mixtures
are
a
management
strategy
relying
on
increases
in
environmental
complexity
to
provide
better
control
of
resistance.
The
results
presented
show
that
mixtures
were
effective
at
slowing
the
evolution
of
resistance
when
all
mixture
components
were
used
at
fully
effective
doses,
while
low
doses
of
mixtures
accelerated
resistance
evolution
and
led
to
more
cross-‐resistance.
Finally,
modifications
of
the
applied
herbicide
dose
allowed
the
study
of
local
adaptation
along
an
environmental
gradient,
where
the
differences
in
outcomes
based
on
the
specific
herbicides
used
were
again
evident.
Overall,
the
work
presented
here
uses
applied
scenarios
to
study
the
underlying
evolutionary
phenomena,
in
order
to
feed
back
into
the
applied
thinking.
Item Type: | Thesis (PhD) | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QK Botany | ||||
Library of Congress Subject Headings (LCSH): | Chlamydomonas reinhardtii, Herbicide resistance | ||||
Official Date: | October 2012 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | School of Life Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Neve, Paul; Colegrave, Nick | ||||
Sponsors: | Leverhulme Trust (LT) | ||||
Extent: | v, 114 leaves : illustrations, charts. | ||||
Language: | eng |
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