Read, D. S., Woodcock, D. J., Strachan, N. J. C., Forbes, K. J., Colles, F. M., Maiden, M. C. J., Clifton-Hadley, F., Ridley, A., Vidal, A., Rodgers, J., Whiteley, A. S. and Sheppard, S. K. (2013) Evidence for phenotypic plasticity among multihost campylobacter jejuni and C. coli lineages, obtained using ribosomal multilocus sequence typing and raman Spectroscopy. Applied and Environmental Microbiology, Volume 79 (Number 3). pp. 965-973. doi:10.1128/AEM.02521-12 ISSN 0099-2240.

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Abstract

Closely related bacterial isolates can display divergent phenotypes. This can limit the usefulness of phylogenetic studies for understanding bacterial ecology and evolution. Here, we compare phenotyping based on Raman spectrometric analysis of cellular composition to phylogenetic classification by ribosomal multilocus sequence typing (rMLST) in 108 isolates of the zoonotic pathogens Campylobacter jejuni and C. coli. Automatic relevance determination (ARD) was used to identify informative peaks in the Raman spectra that could be used to distinguish strains in taxonomic and host source groups (species, clade, clonal complex, and isolate source/host). Phenotypic characterization based on Raman spectra showed a degree of agreement with genotypic classification using rMLST, with segregation accuracy between species (83.95%), clade (in C. coli, 98.41%), and, to some extent, clonal complex (86.89% C. jejuni ST-21 and ST-45 complexes) being achieved. This confirmed the utility of Raman spectroscopy for lineage classification and the correlation between genotypic and phenotypic classification. In parallel analysis, relatively distantly related isolates (different clonal complexes) were assigned the correct host origin irrespective of the clonal origin (74.07 to 96.97% accuracy) based upon different Raman peaks. This suggests that the phenotypic characteristics, from which the phenotypic signal is derived, are not fixed by clonal descent but are influenced by the host environment and change as strains move between hosts.

Item Type:	Journal Article
Divisions:	Faculty of Science, Engineering and Medicine > Research Centres > Warwick Systems Biology Centre
Journal or Publication Title:	Applied and Environmental Microbiology
Publisher:	American Society for Microbiology
ISSN:	0099-2240
Official Date:	February 2013
Dates:	Date Event February 2013 Published
Volume:	Volume 79
Number:	Number 3
Page Range:	pp. 965-973
DOI:	10.1128/AEM.02521-12
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access

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