Clarke, Ian Nicholas (1982) Genomic variation in rotaviruses. PhD thesis, University of Warwick.

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Abstract

The rotaviruses are a recently defined ubiquitous group of
viruses responsible
for
causing acute-gastroenteritis
in human infants
and young animals. Biochemical studies have shown that the rotavirus
genome consists of
11
segments of double-stranded RNA (dsRNA).
This thesis concerns an
investigation
of
the nature and extent
of genomic variation
in
rötaviruses. A rapid and sensitive method
for analyzing the genome profiles of rotavirus
field isolates
was
developed. This is based
on
the direct extraction of dsRNA from
faecal
samples followed by radiolabelling with
[32P3
pCp using
T4
RNA ligase. This
procedure
has been further developed to produce a
method for
generating diagnostic fingerprints from individual species
of dsRNA.

A detailed
structural study making use of this fingerprinting
method has been
undertaken on bovine, porcine and human rotavirus
isolates.
These
analyses show
that genome segment mobility variations
are always associated with detectable changes
in
nucleotide sequence.
They
also show that corresponding genome segments with no mobility
variation can have
sequence-changes at
least
as great as
those found
in
segments
showing electrophoretic mobility variation.
These results
also revealed evidence for
genome segment specific regions of
terminal
sequence conservation.
Evidence
for the
occurrence of genome segment reassortment
between
viruses in the field
was obtained.
Finally
evidence
for the existence of a
'new'
porcine rotavirus
which is
antigenically unrelated
to previously
described
rotaviruses
and has
an unusual pattern
for it's 11
genome segments is presented.

Item Type:	Thesis (PhD)
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology
Library of Congress Subject Headings (LCSH):	Rotaviruses, Genomes
Official Date:	October 1982
Dates:	Date Event October 1982 Submitted
Institution:	University of Warwick
Theses Department:	Department of Biological Sciences
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	McCrae, M. A.
Extent:	xix, 299 leaves : illustrations
Language:	enk

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