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CRISPR typing and subtyping for improved laboratory surveillance of salmonella infections
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Fabre, Laëtitia, Zhang, Jian, Guigon, Ghislaine, Le Hello, Simon, Guibert, Véronique, Accou-Demartin, Marie, de Romans, Saïana, Lim, Catherine, Roux, Chrystelle, Passet, Virginie, Diancourt, Laure, Guibourdenche, Martine, Issenhuth-Jeanjean, Sylvie, Achtman, Mark, Brisse, Sylvain, Sola, Christophe and Weill, François-Xavier (2012) CRISPR typing and subtyping for improved laboratory surveillance of salmonella infections. PLoS One, Volume 7 (Number 5). Article number e36995. doi:10.1371/journal.pone.0036995 ISSN 1932-6203.
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WRAP_journal.pone.0036995.pdf - Published Version Available under License Creative Commons Attribution. Download (916Kb) | Preview |
Official URL: http://dx.doi.org/10.1371/journal.pone.0036995
Abstract
Laboratory surveillance systems for salmonellosis should ideally be based on the rapid serotyping and subtyping of isolates. However, current typing methods are limited in both speed and precision. Using 783 strains and isolates belonging to 130 serotypes, we show here that a new family of DNA repeats named CRISPR (clustered regularly interspaced short palindromic repeats) is highly polymorphic in Salmonella. We found that CRISPR polymorphism was strongly correlated with both serotype and multilocus sequence type. Furthermore, spacer microevolution discriminated between subtypes within prevalent serotypes, making it possible to carry out typing and subtyping in a single step. We developed a high-throughput subtyping assay for the most prevalent serotype, Typhimurium. An open web-accessible database was set up, providing a serotype/spacer dictionary and an international tool for strain tracking based on this innovative, powerful typing and subtyping tool.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine |
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| Library of Congress Subject Headings (LCSH): | Salmonella food poisoning, Salmonella infections , Salmonella -- Detection, Salmonella -- Genetics | ||||
| Journal or Publication Title: | PLoS One | ||||
| Publisher: | Public Library of Science | ||||
| ISSN: | 1932-6203 | ||||
| Official Date: | 2012 | ||||
| Dates: |
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| Volume: | Volume 7 | ||||
| Number: | Number 5 | ||||
| Page Range: | Article number e36995 | ||||
| DOI: | 10.1371/journal.pone.0036995 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||
| Description: | Formal Correction: This article has been formally corrected to address the following errors. There were errors in Tables 1, 3, 5, 7, and 8. Footnote citations were missing from Table 1, 5, and 7. Text was missing from Table 3. There were formatting errors in Table 8. The corrected tables can be viewed here: Table 1:... (read formal correction) |
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| Date of first compliant deposit: | 26 December 2015 | ||||
| Date of first compliant Open Access: | 26 December 2015 | ||||
| Funder: | Institut Pasteur (Paris, France), Institut de Veille Sanitaire (Saint Maurice, France) (INVS), Science Foundation Ireland (SFI) | ||||
| Grant number: | 05/FE1/B882 (SFI) |
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