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Complementation of a speA negative Streptococcus pyogenes with speA : effects on virulence and production of streptococcal pyrogenic exotoxin A
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Unnikrishnan, Meera, Cohen, Jonathan and Sriskandan, Shiranee (2001) Complementation of a speA negative Streptococcus pyogenes with speA : effects on virulence and production of streptococcal pyrogenic exotoxin A. Microbial Pathogenesis, Volume 31 (Number 3). pp. 109-114. doi:10.1006/mpat.2001.0453 ISSN 0882-4010.
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Official URL: http://dx.doi.org/10.1006/mpat.2001.0453
Abstract
We have shown previously that an isogenic SPEA-negative Streptococcus pyogenes strain did not attenuate virulence in a murine model of necrotizing fasciitis. The aim of this study was to confirm that streptococcal pyrogenic exotoxin A (SPEA) is not crucial for streptococcal invasiveness in murine invasive infection. The SPEA-negative S. pyogenes (H326) was complemented with speA extra-chromosomally to create strain H361 which produced 2.2-fold more SPEA compared with the parental speA+wild-type (H305). The growth phase-regulated expression of SPEAin vitro was unaffected in this strain. Complementation with speA resulted in reduced virulence and bacterial counts in invasive murine infection. SPEA production was quantitated from muscle tissue of infected mice. However, H361 did not produce more SPEA than H305 in vivo. We conclude that SPEA does not play a key role in invasive murine streptococcal infection.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Streptococcus pyogenes, Superantigens, Virulence (Microbiology) | ||||
Journal or Publication Title: | Microbial Pathogenesis | ||||
Publisher: | Academic Press | ||||
ISSN: | 0882-4010 | ||||
Official Date: | September 2001 | ||||
Dates: |
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Volume: | Volume 31 | ||||
Number: | Number 3 | ||||
Page Range: | pp. 109-114 | ||||
DOI: | 10.1006/mpat.2001.0453 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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